@article{3ae39efc9229444981a5b65e5cc2e808,
title = "Human Antibodies Protect against Aerosolized Eastern Equine Encephalitis Virus Infection",
abstract = "Eastern equine encephalitis virus (EEEV) causes rare but serious and often fatal infection associated with inflammation of the brain. Monoclonal antibodies identified from a human survivor recognize distinct epitopes to prevent viral entry recognize, demonstrate protection as well as potent neutralization against EEEV, and may inform future vaccine design.",
keywords = "Eastern equine, aerosol, encephalitis, human antibodies, monoclonal, neutralizing, prophylaxis, therapy",
author = "Williamson, {Lauren E.} and Theron Gilliland and Yadav, {Pramod K.} and Elad Binshtein and Robin Bombardi and Nurgun Kose and Nargi, {Rachel S.} and Sutton, {Rachel E.} and Durie, {Clarissa L.} and Erica Armstrong and Carnahan, {Robert H.} and Walker, {Lauren M.} and Kim, {Arthur S.} and Fox, {Julie M.} and Diamond, {Michael S.} and Ohi, {Melanie D.} and Klimstra, {William B.} and Crowe, {James E.}",
note = "Funding Information: We thank J.D. Laughlin for performing Octet HTX experiments. We thank P. Gilchuk, C. Slaughter, M. Vogt, and S. Zost at Vanderbilt for helpful discussions and training. We thank D. Yousif, M. Jensen, N. Murphy, M. Goff, I. Kovach, J. Rodriguez, C. Gainza, W. Reichard, M. Majedi, J. Reidy, A. Trivette, and R. Troseth for technical support. We thank M. Leksell, M. Mayo, K. Moton, T. Martin, V. Morris, A. Bunnell, and A. Jordan for administrative assistance and R. Irving for lab management. The University of Michigan Cryo-EM Facility has received support from the U-M Life Sciences Institute and the U-M Biosciences Initiative . We acknowledge M. Su, A. Bondy, L. Keopping, C. Lilienthal, and B. Battey for U-M cryo-EM technical and IT support. Cryo-EM computation done at the LSI was supported by NIH ( S10OD020011 ). EM data collections of EEEV VLP and EEEV VLP:rEEEV-143 Fab were conducted at the Center for Structural Biology Cryo-EM facility at Vanderbilt University. L.E.W. was supported by NIH ( T32 HL069765 and F31 AI145189 ). This project received support from the U.S. Defense Threat Reduction Agency ( HDTRA1-13-1-0034 to J.E.C. and HDTRA1-15-1-0047 to W.B.K.), NIH ( U19 AI142790 to J.E.C. and M.S.D. and R01 AI095436 to W.B.K.), and by CTSA UL1 TR002243 from the U.S. NCATS . The graphical abstract was made in part with tools at Biorender.com. Funding Information: We thank J.D. Laughlin for performing Octet HTX experiments. We thank P. Gilchuk, C. Slaughter, M. Vogt, and S. Zost at Vanderbilt for helpful discussions and training. We thank D. Yousif, M. Jensen, N. Murphy, M. Goff, I. Kovach, J. Rodriguez, C. Gainza, W. Reichard, M. Majedi, J. Reidy, A. Trivette, and R. Troseth for technical support. We thank M. Leksell, M. Mayo, K. Moton, T. Martin, V. Morris, A. Bunnell, and A. Jordan for administrative assistance and R. Irving for lab management. The University of Michigan Cryo-EM Facility has received support from the U-M Life Sciences Institute and the U-M Biosciences Initiative. We acknowledge M. Su, A. Bondy, L. Keopping, C. Lilienthal, and B. Battey for U-M cryo-EM technical and IT support. Cryo-EM computation done at the LSI was supported by NIH (S10OD020011). EM data collections of EEEV VLP and EEEV VLP:rEEEV-143 Fab were conducted at the Center for Structural Biology Cryo-EM facility at Vanderbilt University. L.E.W. was supported by NIH (T32 HL069765 and F31 AI145189). This project received support from the U.S. Defense Threat Reduction Agency (HDTRA1-13-1-0034 to J.E.C. and HDTRA1-15-1-0047 to W.B.K.), NIH (U19 AI142790 to J.E.C. and M.S.D. and R01 AI095436 to W.B.K.), and by CTSA UL1 TR002243 from the U.S. NCATS. The graphical abstract was made in part with tools at Biorender.com. Conceptualization, L.E.W. M.S.D. M.D.O. W.B.K. and J.E.C.; Methodology, L.E.W. W.B.K. M.D.O. and J.E.C.; Investigation, L.E.W. T.G. P.Y. E.B. R.B. N.K. R.S.N. R.E.S. C.L.D. E.A. L.M.W. and J.M.F.; Resources, A.S.K. M.S.D. and W.B.K.; Writing ? Original Draft, L.E.W. and J.E.C.; Writing ? Review & Editing, L.E.W. M.S.D. M.D.O. W.B.K. and J.E.C.; Supervision, R.H.C. M.S.D. W.B.K. M.D.O. and J.E.C.; Project Administration, R.H.C. and J.E.C.; Funding Acquisition, M.S.D. W.B.K. M.D.O. and J.E.C. M.S.D. is consultant for Inbios, Vir Biotechnology, and NGM Biopharmaceuticals, is on the Scientific Advisory Boards of Moderna and Immunome, and is a recipient of grants from Moderna, Vir Biotechnology, and Emergent BioSolutions. J.E.C. has served as consultant for Eli Lilly and Sanofi, is on Scientific Advisory Boards of CompuVax and Meissa Vaccines, is recipient of research grants from Moderna and Sanofi, and is Founder of IDBiologics, Inc. Vanderbilt University has applied for patents that are related to this work. Funding Information: M.S.D. is consultant for Inbios, Vir Biotechnology, and NGM Biopharmaceuticals, is on the Scientific Advisory Boards of Moderna and Immunome, and is a recipient of grants from Moderna, Vir Biotechnology, and Emergent BioSolutions. J.E.C. has served as consultant for Eli Lilly and Sanofi, is on Scientific Advisory Boards of CompuVax and Meissa Vaccines, is recipient of research grants from Moderna and Sanofi, and is Founder of IDBiologics, Inc. Vanderbilt University has applied for patents that are related to this work. Publisher Copyright: {\textcopyright} 2020 Elsevier Inc.",
year = "2020",
month = dec,
day = "23",
doi = "10.1016/j.cell.2020.11.011",
language = "English",
volume = "183",
pages = "1884--1900.e23",
journal = "Cell",
issn = "0092-8674",
number = "7",
}