TY - JOUR
T1 - Human and nonhuman primate meninges harbor lymphatic vessels that can be visualized noninvasively by MRI
AU - Absinta, Martina
AU - Ha, Seung Kwon
AU - Nair, Govind
AU - Sati, Pascal
AU - Luciano, Nicholas J.
AU - Palisoc, Maryknoll
AU - Louveau, Antoine
AU - Zaghloul, Kareem A.
AU - Pittaluga, Stefania
AU - Kipnis, Jonathan
AU - Reich, Daniel S.
N1 - Funding Information:
Martina Absinta: Dr. Absinta was partially supported by a National Multiple Sclerosis Society (NMSS) fellowship award #FG 2093-A-1 and holds a Marilyn Hilton Award for Innovation in MS research from the Conrad N. Hilton Foundation. Daniel S Reich: Dr. Reich received research support from collaborations with the Myelin Repair Foundation and Vertex Pharmaceuticals, unrelated to the present study. The other authors declare that no competing interests exist.
Funding Information:
The Intramural Research Program of NINDS supported this study. We thank the study participants, Irene Cortese and the NINDS Neuroimmunology Clinic for patient recruitment and care, the NIH Functional Magnetic Resonance Imaging Facility for MRI support, Afonso C Silva and his laboratory for access to the 7T animal scanner and customized hardware, Steven Jacobson and Emily Leibovitch for collaboration on our animal studies, and Roger Depaz for assistance in animal preparation.
Publisher Copyright:
© 2017, eLife Sciences Publications Ltd. All rights reserved.
PY - 2017/10/3
Y1 - 2017/10/3
N2 - Here, we report the existence of meningeal lymphatic vessels in human and nonhuman primates (common marmoset monkeys) and the feasibility of noninvasively imaging and mapping them in vivo with high-resolution, clinical MRI. On T2-FLAIR and T1-weighted black-blood imaging, lymphatic vessels enhance with gadobutrol, a gadolinium-based contrast agent with high propensity to extravasate across a permeable capillary endothelial barrier, but not with gadofosveset, a blood-pool contrast agent. The topography of these vessels, running alongside dural venous sinuses, recapitulates the meningeal lymphatic system of rodents. In primates, meningeal lymphatics display a typical panel of lymphatic endothelial markers by immunohistochemistry. This discovery holds promise for better understanding the normal physiology of lymphatic drainage from the central nervous system and potential aberrations in neurological diseases.
AB - Here, we report the existence of meningeal lymphatic vessels in human and nonhuman primates (common marmoset monkeys) and the feasibility of noninvasively imaging and mapping them in vivo with high-resolution, clinical MRI. On T2-FLAIR and T1-weighted black-blood imaging, lymphatic vessels enhance with gadobutrol, a gadolinium-based contrast agent with high propensity to extravasate across a permeable capillary endothelial barrier, but not with gadofosveset, a blood-pool contrast agent. The topography of these vessels, running alongside dural venous sinuses, recapitulates the meningeal lymphatic system of rodents. In primates, meningeal lymphatics display a typical panel of lymphatic endothelial markers by immunohistochemistry. This discovery holds promise for better understanding the normal physiology of lymphatic drainage from the central nervous system and potential aberrations in neurological diseases.
UR - http://www.scopus.com/inward/record.url?scp=85032886612&partnerID=8YFLogxK
U2 - 10.7554/eLife.29738.001
DO - 10.7554/eLife.29738.001
M3 - Article
C2 - 28971799
AN - SCOPUS:85032886612
SN - 2050-084X
VL - 6
JO - eLife
JF - eLife
M1 - e29738
ER -