Human airway epithelial cells present antigen to influenza virus-specific CD8+ CTL inefficiently after incubation with viral protein together with ISCOMATRIX®

G. F. Rimmelzwaan, A. C.M. Boon, M. M. Geelhoed-Mieras, J. T.M. Voeten, R. A.M. Fouchier, A. D.M.E. Osterhaus

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

In the present paper, an in vitro model was established in which the interaction between influenza virus-specific CD8+ T cells and human airway epithelial cells can be studied. To this end, the human lung epithelial cell line A549 was transduced with the HLA-A*0201 gene. This MHC class I allele is involved in the presentation of the immunodominant M158-66 cytotoxic T lymphocyte (CTL) epitope of the influenza A virus matrix protein. The A549-HLA-A2 cells and a CD8+ T cell clone specific for the M1 58-66 epitope were used to evaluate ISCOMATRIX® (IMX), which is considered a potential mucosal adjuvant for influenza vaccines, for its capacity to activate virus-specific CTL after incubation with epithelial cells. It was found that virus infected epithelial cells activated virus-specific CTL efficiently. However, incubation of epithelial cells with ISCOMATRIX® and recombinant M1 protein activated CD8+ T cells inefficiently, unlike the incubation of C1R cells expressing a HLA-A2 trans gene or HLA-A2+ B-lymphoblastoid cells with these reagents. It was concluded that this lack of antigen presentation by epithelial cells indicate that these cells are not subject to killing by virus-specific CTL upon instillation with ISCOMATRIX®-based vaccines, which may be a favorable property of mucosal vaccines.

Original languageEnglish
Pages (from-to)2769-2775
Number of pages7
JournalVaccine
Volume22
Issue number21-22
DOIs
StatePublished - Jul 29 2004
Externally publishedYes

Keywords

  • ISCOMATRIX®
  • Influenza virus
  • Vaccine

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