Homophilic Dscam Interactions Control Complex Dendrite Morphogenesis

Michael E. Hughes, Rachel Bortnick, Asako Tsubouchi, Philipp Bäumer, Masahiro Kondo, Tadashi Uemura, Dietmar Schmucker

Research output: Contribution to journalArticle

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Alternative splicing of the Drosophila gene Dscam results in up to 38,016 different receptor isoforms proposed to interact by isoform-specific homophilic binding. We report that Dscam controls cell-intrinsic aspects of dendrite guidance in all four classes of dendrite arborization (da) neurons. Loss of Dscam in single neurons causes a strong increase in self-crossing. Restriction of dendritic fields of neighboring class III neurons appeared intact in mutant neurons, suggesting that dendritic self-avoidance, but not heteroneuronal tiling, may depend on Dscam. Overexpression of the same Dscam isoforms in two da neurons with overlapping dendritic fields forced a spatial segregation of the two fields, supporting the model that dendritic branches of da neurons use isoform-specific homophilic interactions to ensure minimal overlap. Homophilic binding of the highly diverse extracellular domains of Dscam may therefore limit the use of the same "core" repulsion mechanism to cell-intrinsic interactions without interfering with heteroneuronal interactions.

Original languageEnglish
Pages (from-to)417-427
Number of pages11
Issue number3
StatePublished - May 3 2007
Externally publishedYes



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    Hughes, M. E., Bortnick, R., Tsubouchi, A., Bäumer, P., Kondo, M., Uemura, T., & Schmucker, D. (2007). Homophilic Dscam Interactions Control Complex Dendrite Morphogenesis. Neuron, 54(3), 417-427. https://doi.org/10.1016/j.neuron.2007.04.013