The domain of HIV-2 Vpx previously shown to be important for virion incorporation has been mapped to residues 73-89. Mutational analysis of this domain was employed to further define the sequences important for incorporation into virus-like particles, using a vaccinia virus expression system. Deletion of residues 73-89 did not abrogate Vpx packaging, but substitution with alanines markedly reduced incorporation into virus-like particles. Moreover, alanine substitution also disrupted Vpx interaction with Gag, as demonstrated with glutathione S-transferase fusion proteins and the yeast two-hybrid system.

Original languageEnglish
Pages (from-to)105-111
Number of pages7
JournalAIDS research and human retroviruses
Issue number2
StatePublished - 2001


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