HIV-1 Integrase Binds the Viral RNA Genome and Is Essential during Virion Morphogenesis

Jacques J. Kessl, Sebla B. Kutluay, Dana Townsend, Stephanie Rebensburg, Alison Slaughter, Ross C. Larue, Nikoloz Shkriabai, Nordine Bakouche, James R. Fuchs, Paul D. Bieniasz, Mamuka Kvaratskhelia

Research output: Contribution to journalArticlepeer-review

90 Scopus citations


While an essential role of HIV-1 integrase (IN) for integration of viral cDNA into human chromosome is established, studies with IN mutants and allosteric IN inhibitors (ALLINIs) have suggested that IN can also influence viral particle maturation. However, it has remained enigmatic as to how IN contributes to virion morphogenesis. Here, we demonstrate that IN directly binds the viral RNA genome in virions. These interactions have specificity, as IN exhibits distinct preference for select viral RNA structural elements. We show that IN substitutions that selectively impair its binding to viral RNA result in eccentric, non-infectious virions without affecting nucleocapsid-RNA interactions. Likewise, ALLINIs impair IN binding to viral RNA in virions of wild-type, but not escape mutant, virus. These results reveal an unexpected biological role of IN binding to the viral RNA genome during virion morphogenesis and elucidate the mode of action of ALLINIs.

Original languageEnglish
Pages (from-to)1257-1268.e12
Issue number5
StatePublished - Aug 25 2016


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