HIV-1 determinants of thrombocytopenia at the stage of CD34+ progenitor cell differentiation in vivo lie in the viral envelope gp120 V3 loop region

Menghua Zhang, Stella Evans, Jinyun Yuan, Lee Ratner, Prasad S. Koka

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

HIV-1 V3 loop clones of virus isolates derived from patients suffering from thrombocytopenia were used for infection of the human thymus/liver conjoint hematopoietic organ that developed in the severe combined immunodeficient mouse (SCID-hu Thy/Liv). The V3 loop clones showed a significantly greater degree of inhibition of megakaryopoiesis than myelopoiesis and erythropoiesis of the human CD34+ progenitor cells, in vivo. Inhibition of megakaryopoiesis occurs through reduction in c-Mpl expression and consequent decrease in STAT5 activation. Therefore HIV-1 V3 loop sequences of thrombocytopenic patients exhibit preferential inhibition of megakaryocyte lineage-specific differentiation of CD34+ progenitor cells, thus reflecting the patients' clinical condition.

Original languageEnglish
Pages (from-to)131-136
Number of pages6
JournalVirology
Volume401
Issue number2
DOIs
StatePublished - Jun 2010

Keywords

  • CFU-MK
  • HIV-1
  • Hematopoiesis
  • SCID-hu
  • V3 loop

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