TY - JOUR
T1 - Histone acetylation favours the cardiovascular commitment of adipose tissue-derived stromal cells
AU - De Falco, Elena
AU - Bordin, Antonella
AU - Scaccia, Eleonora
AU - Pagano, Francesca
AU - Ibrahim, Mohsen
AU - Schirone, Leonardo
AU - Angelini, Francesco
AU - Palmerio, Silvia
AU - Madonna, Michele
AU - Fianchini, Luca
AU - Chimenti, Isotta
AU - Sciarretta, Sebastiano
AU - Frati, Giacomo
N1 - Funding Information:
We thank Fondazione Roma. This study was financially supported by the Sapienza University of Rome, with a grant to Elena De Falco (grant number C26A1429M2). The sponsor had no involvement in the collection, analysis and interpretation of data, in the writing of the report and in the decision to submit the article for publication.
Publisher Copyright:
© 2017 Elsevier Ireland Ltd
PY - 2017/9/15
Y1 - 2017/9/15
N2 - Background Although adipose stromal cells (ASCs) retain the ability to transdifferentiate at low rate towards the cardiac lineage, the potential mechanisms underlying such process have still to be elucidated. Methods Since chromatin state modifications are involved in several processes regulating the cellular cell fate commitment, we aimed at evaluating the role of histone protein acetylation in the cardiovascular-like transdifferentiation of ASCs. Results We found a clear increase of histone 3 acetylation status paralleled by a significant upregulation of cardiac TnI gene expression, in ASCs treated with the conditioned medium of primary cardiomyocyte cell cultures for 72 h. This result suggests that histone acetylation contributes to the transdifferentiation of ASCs towards the cardiac lineage. In order to directly test this hypothesis, ASCs cultured with regular medium were treated with SAHA, a pan histone deacetylase inhibitor. We found that SAHA enhanced the cardiac permissive state of ASCs, increasing both mRNA and protein expression of cardiovascular genes, particularly cTnI. This suggests that histone acetylation induction is sufficient to promote cardiovascular transdifferentiation. Conclusions The control of ASC fate by epigenetic regulators might be an interesting tool to boost both cardiac commitment and regenerative capacities of ASCs.
AB - Background Although adipose stromal cells (ASCs) retain the ability to transdifferentiate at low rate towards the cardiac lineage, the potential mechanisms underlying such process have still to be elucidated. Methods Since chromatin state modifications are involved in several processes regulating the cellular cell fate commitment, we aimed at evaluating the role of histone protein acetylation in the cardiovascular-like transdifferentiation of ASCs. Results We found a clear increase of histone 3 acetylation status paralleled by a significant upregulation of cardiac TnI gene expression, in ASCs treated with the conditioned medium of primary cardiomyocyte cell cultures for 72 h. This result suggests that histone acetylation contributes to the transdifferentiation of ASCs towards the cardiac lineage. In order to directly test this hypothesis, ASCs cultured with regular medium were treated with SAHA, a pan histone deacetylase inhibitor. We found that SAHA enhanced the cardiac permissive state of ASCs, increasing both mRNA and protein expression of cardiovascular genes, particularly cTnI. This suggests that histone acetylation induction is sufficient to promote cardiovascular transdifferentiation. Conclusions The control of ASC fate by epigenetic regulators might be an interesting tool to boost both cardiac commitment and regenerative capacities of ASCs.
KW - Adipose stromal cells
KW - Cardiac Troponin I
KW - Cardiac transdifferentiation
KW - Cardiomyocytes
KW - Histone 3
KW - SAHA
UR - http://www.scopus.com/inward/record.url?scp=85020393338&partnerID=8YFLogxK
U2 - 10.1016/j.ijcard.2017.05.112
DO - 10.1016/j.ijcard.2017.05.112
M3 - Article
C2 - 28610961
AN - SCOPUS:85020393338
SN - 0167-5273
VL - 243
SP - 421
EP - 423
JO - International Journal of Cardiology
JF - International Journal of Cardiology
ER -