Histochemical evidence for induction of arachidonate 15-lipoxygenase in airway disease

V. R. Shannon, P. Chanez, J. Bousquet, M. J. Holtzman

Research output: Contribution to journalArticle

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Abstract

We have previously described the distribution of the arachidonate 15- lipoxygenase in lung tissue obtained from healthy human subjects. In the present study, we have utilized the same immunohistochemical methodology to examine the expression of 15-lipoxygenase in bronchial biopsy tissue from subjects with airway disease. Immunohistochemistry of bronchial tissue using two antibodies against distinct epitopes of the 15-lipoxygenase and indirect biotin-avidin-peroxidase detection demonstrated that, in contrast to airway tissue from normal subjects (n = 10) in which 15-lipoxygenase antigen was confined to the uppermost airways (nose and trachea) and was almost undetectable in bronchi, the bronchial tissue obtained from subjects with asthma (n = 7) or chronic bronchitis (n = 7) exhibited markedly positive immunostaining of mucosal epithelial cells with both anti-15-lipoxygenase antibodies. Specificity of 15-lipoxygenase immunostaining was verified by antigen competition experiments and by the lack of immunostaining with preimmune serum or control anti-5-lipoxygenase antibodies. The increased levels of 15-lipoxygenase antigen in the bronchial epithelial cells of asthmatic and bronchitic subjects compared with the same cell population in normal subjects coupled with the previous findings of increased 15- lipoxygenase activity in asthmatic airways suggest that epithelial 15- lipoxygenase is induced by airway inflammatory disease.

Original languageEnglish
Pages (from-to)1024-1028
Number of pages5
JournalAmerican Review of Respiratory Disease
Volume147
Issue number4
DOIs
StatePublished - Jan 1 1993

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