Histamine H3 receptor-mediated suppression of inhibitory synaptic transmission in the submucous plexus of guinea-pig small intestine

Sumei Liu, Yun Xia, Hong Zhen Hu, Jun Ren, Chuanyun Gao, Jackie D. Wood

Research output: Contribution to journalArticlepeer-review

46 Scopus citations

Abstract

Conventional intracellular microelectrodes and marker injection techniques were used to study the actions of histamine on inhibitory synaptic transmission in the submucous plexus of guinea-pig small intestine. Bath application of histamine (1-300 μM) reversibly suppressed both noradrenergic and non-adrenergic slow inhibitory postsynaptic potentials in a concentration-dependent manner. These effects of histamine were mimicked by the selective histamine H3 receptor agonist R(-)-α-methylhistamine but not the selective histamine H1 receptor agonist, 6-[2-(4-imidazolyl)ethylamino]-N-(4-trifluoromethylphenyl) heptanecarboxamide (HTMT dimaleate), or the selective histamine H2 receptor agonist, dimaprit. The histamine H3 receptor antagonist, thioperamide, blocked the effects of histamine. Histamine H1 and H2 receptor antagonists did not change the action of histamine. Hyperpolarizing responses to focal application of norepinephrine or somatostatin by pressure ejection from micropipettes were unaffected by histamine and R(-)-α-methylhistamine. The results suggest that histamine acts at presynaptic histamine H3 receptors on the terminals of sympathetic postganglionic fibers and intrinsic somatostatinergic nerves in the small intestine to suppress the release of the inhibitory neurotransmitters, norepinephrine and somatostatin. (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)49-54
Number of pages6
JournalEuropean Journal of Pharmacology
Volume397
Issue number1
DOIs
StatePublished - May 26 2000

Keywords

  • Enteric nervous system
  • Norepinephrine
  • Small intestine
  • Somatostatin
  • Submucous plexus
  • Synaptic transmission
  • inhibitory

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