Hindering auxiliary anchors are potent modulators of peptide binding and selection by I-A(k) class II molecules

R. R. Latek, S. J. Petzold, E. R. Unanue

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

Selection of particular antigen-derived peptides by class II MHC molecules determines the population of complexes represented on the antigen-presenting cell surface and available for T cell receptor engagement. This discriminating selection process results from unique interactions between the spectrum of peptides generated during antigen processing and the MHC molecules. Here, we examined the selection of peptides by the class II MHC, I-A(k). Our results indicate that although peptide primary anchors are key in MHC binding, auxiliary anchors are a powerful regulatory component in the selection of peptides by I-A(k). Study of the segments surrounding the dominant hen egg white lysozome(48-61) epitope demonstrates that auxiliary anchors also are involved in determining the binding register of I-A(k) along an extended peptide. In addition, we found that unique combinations of auxiliary anchors can act in concert to modulate the binding of peptides to I-A(k).

Original languageEnglish
Pages (from-to)11460-11465
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume97
Issue number21
DOIs
StatePublished - Oct 10 2000

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