TY - JOUR
T1 - Highwire regulates synaptic growth in Drosophila
AU - Wan, Hong I.
AU - DiAntonio, Aaron
AU - Fetter, Richard D.
AU - Bergstrom, Kendra
AU - Strauss, Roland
AU - Goodman, Corey S.
N1 - Funding Information:
We thank Karen Zito, Graeme Davis, and Sophie Petersen for help with physiology; Dorit Parnas, Hermann Alberle, Brian McCabe, and Pejmun Haghighi for critical reading of the manuscript; Jack Roos and Regis Kelly for the Dap160 antiserum; Larry Zipursky for the Pak antiserum; Hugo Bellen for the Syt antiserum; and members of the Goodman laboratory for advice throughout this study. We thank Chihiro Hama and colleagues for sharing their results on SIF and Fas II prior to publication, and Yishi Jin, Mike Nonet, and their colleagues for sharing their results on RPM-1 prior to publication. H. I. W. was supported by National Institutes of Health grant HD-21294 to C. S. G. A. D. was supported by a Helen Hay Whiney Postdoctoral Fellowship and a Burroughs Wellcome Career Award. R. S. was supported by the Max-Planck-Gesellschaft. R. D. F. is a Senior Research Associate, and C. S. G. is an Investigator, with the Howard Hughes Medical Institute.
PY - 2000
Y1 - 2000
N2 - The formation, stabilization, and growth of synaptic connections are dynamic and highly regulated processes. The glutamatergic neuromuscular junction (NMJ) in Drosophila grows new boutons and branches throughout larval development. A primary walking behavior screen followed by a secondary anatomical screen led to the identification of the highwire (hiw) gene. In hiw mutants, the specificity of motor axon pathfinding and synapse formation appears normal. However, NMJ synapses grow exuberantly and are greatly expanded in both the number of boutons and the extent and length of branches. These synapses appear normal ultrastructurally but have reduced quantal content physiologically. hiw encodes a large protein found at presynaptic terminals. Within presynaptic terminals, HIW is localized to the periactive zone surrounding active zones; Fasciclin II (Fas II), which also controls synaptic growth, is found at the same location.
AB - The formation, stabilization, and growth of synaptic connections are dynamic and highly regulated processes. The glutamatergic neuromuscular junction (NMJ) in Drosophila grows new boutons and branches throughout larval development. A primary walking behavior screen followed by a secondary anatomical screen led to the identification of the highwire (hiw) gene. In hiw mutants, the specificity of motor axon pathfinding and synapse formation appears normal. However, NMJ synapses grow exuberantly and are greatly expanded in both the number of boutons and the extent and length of branches. These synapses appear normal ultrastructurally but have reduced quantal content physiologically. hiw encodes a large protein found at presynaptic terminals. Within presynaptic terminals, HIW is localized to the periactive zone surrounding active zones; Fasciclin II (Fas II), which also controls synaptic growth, is found at the same location.
UR - http://www.scopus.com/inward/record.url?scp=0033715810&partnerID=8YFLogxK
U2 - 10.1016/S0896-6273(00)81166-6
DO - 10.1016/S0896-6273(00)81166-6
M3 - Article
C2 - 10839352
AN - SCOPUS:0033715810
SN - 0896-6273
VL - 26
SP - 313
EP - 329
JO - Neuron
JF - Neuron
IS - 2
ER -