High-throughput, liquid-based genome-wide RNAi screening in C. Elegans

Linda P. O’Reilly, Ryan R. Knoerdel, Gary A. Silverman, Stephen C. Pak

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

3 Scopus citations

Abstract

RNA interference (RNAi) is a process in which double-stranded RNA (dsRNA) molecules mediate the inhibition of gene expression. RNAi in C. elegans can be achieved by simply feeding animals with bacteria expressing dsRNA against the gene of interest. This “feeding” method has made it possible to conduct genome-wide RNAi experiments for the systematic knockdown and subsequent investigation of almost every single gene in the genome. Historically, these genome-scale RNAi screens have been labor and time intensive. However, recent advances in automated, high-throughput methodologies have allowed the development of more rapid and efficient screening protocols. In this report, we describe a fast and efficient, liquid-based method for genome-wide RNAi screening.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages151-162
Number of pages12
DOIs
StatePublished - Jan 1 2016
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1470
ISSN (Print)1064-3745

Keywords

  • Arrayscan
  • C. elegans
  • Genome wide
  • High-throughput screening
  • RNAi

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