High-throughput lens-free blood analysis on a chip

Sungkyu Seo, Serhan O. Isikman, Ikbal Sencan, Onur Mudanyali, Ting Wei Su, Waheb Bishara, Anthony Erlinger, Aydogan Ozcan

Research output: Contribution to journalArticlepeer-review

128 Scopus citations

Abstract

We present a detailed investigation of the performance of lens-free holographic microscopy toward high-throughput on-chip blood analysis. Using a spatially incoherent source that is emanating from a large aperture, automated counting of red blood cells with minimal sample preparation steps at densities reaching up to ∼0.4 - 106 cells/μL is presented. Using the same lens-free holographic microscopy platform, we also characterize the volume of the red blood cells at the single-cell level through recovery of the optical phase information of each cell. We further demonstrate the measurement of the hemoglobin concentration of whole blood samples as well as automated counting of white blood cells, also yielding spatial resolution at the subcellular level sufficient to differentiate granulocytes, monocytes, and lymphocytes from each other. These results uncover the prospects of lens-free holographic on-chip imaging to provide a useful tool for global health problems, especially by facilitating whole blood analysis in resource-poor environments.

Original languageEnglish
Pages (from-to)4621-4627
Number of pages7
JournalAnalytical Chemistry
Volume82
Issue number11
DOIs
StatePublished - Jun 1 2010

Fingerprint

Dive into the research topics of 'High-throughput lens-free blood analysis on a chip'. Together they form a unique fingerprint.

Cite this