TY - JOUR
T1 - High-throughput determination of oil content in corn kernels using nuclear magnetic resonance Imaging
AU - Kotyk, John J.
AU - Pagel, Marty D.
AU - Deppermann, Kevin L.
AU - Colletti, Ronald F.
AU - Hoffman, Norman G.
AU - Yannakakis, Elias J.
AU - Das, Pradip K.
AU - Ackerman, Joseph J.H.
N1 - Funding Information:
Early experiments providing preliminary data supporting the utility of the methods described herein were performed at the Biomedical MR Laboratory (BMRL) of Washington University in St. Louis. In recognition of this support, we gratefully acknowledge the assistance and consultation of Joseph J.H. Ackerman and other members of the BMRL. The authors thank Glenn Foster of the Washington University School of Medicine, and Michael Crowley of the St. Louis Children’s Hospital, for helpful discussions and access to clinical MRI instrumentation. A special thanks goes to Ross Braught for assistance with sample preparation and data acquisition. This work was supported through funding from Monsanto Company and Renessen, Inc.
PY - 2005
Y1 - 2005
N2 - A new high-throughput method for measuring oil content in intact, single corn kernels is demonstrated using nuclear magnetic resonance imaging (MRI) methods. This nonde-structive technique enables the evaluation of relative oil content in up to 2,592 corn kernels in less than 40 min using a 1.5 T clinical MRI scanner. Custom software was developed to process and analyze 3-D magnetic resonance (MR) image data rapidly. The precision and accuracy of the MR method for measuring oil content are discussed. The precision of the MRI results is shown to be dependent on MR scanner noise. The MRI results show very good relative accuracy compared with low-field NMR, NIR transmission, and accelerated solvent extraction measurements. Minor differences between the MRI and low-field NMR experimental protocols were shown to be inconsequential to the oil content measurement. Extending the MRI method to the analysis of other oilseeds and/or the use of other magnetic field strengths is discussed, as is a comparison of this MRI method relative to other high-throughput magnetic resonance screening techniques.
AB - A new high-throughput method for measuring oil content in intact, single corn kernels is demonstrated using nuclear magnetic resonance imaging (MRI) methods. This nonde-structive technique enables the evaluation of relative oil content in up to 2,592 corn kernels in less than 40 min using a 1.5 T clinical MRI scanner. Custom software was developed to process and analyze 3-D magnetic resonance (MR) image data rapidly. The precision and accuracy of the MR method for measuring oil content are discussed. The precision of the MRI results is shown to be dependent on MR scanner noise. The MRI results show very good relative accuracy compared with low-field NMR, NIR transmission, and accelerated solvent extraction measurements. Minor differences between the MRI and low-field NMR experimental protocols were shown to be inconsequential to the oil content measurement. Extending the MRI method to the analysis of other oilseeds and/or the use of other magnetic field strengths is discussed, as is a comparison of this MRI method relative to other high-throughput magnetic resonance screening techniques.
KW - High-throughput
KW - Intact seed
KW - MRI
KW - Magnetic resonance imaging
KW - Nondestructive
KW - Proximate oil
UR - http://www.scopus.com/inward/record.url?scp=33947358264&partnerID=8YFLogxK
U2 - 10.1007/s11746-005-1155-5
DO - 10.1007/s11746-005-1155-5
M3 - Article
AN - SCOPUS:33947358264
SN - 0003-021X
VL - 82
SP - 855
EP - 862
JO - JAOCS, Journal of the American Oil Chemists' Society
JF - JAOCS, Journal of the American Oil Chemists' Society
IS - 12
ER -