TY - GEN
T1 - High-throughput blood analysis on a chip using lensless digital holography
AU - Isikman, Serhan O.
AU - Seo, Sungkyu
AU - Sencan, Ikbal
AU - Mudanyali, Onur
AU - Su, Ting Wei
AU - Bishara, Waheb
AU - Erlinger, Anthony
AU - Ozcan, Aydogan
PY - 2010
Y1 - 2010
N2 - We present a lensfree holographic microscopy platform toward high-throughput on-chip blood analysis, which may facilitate point-of-care diagnostics in resource-poor environments. In this lensfree imaging modality, whole blood samples are illuminated with a spatially incoherent light source to record holographic diffraction patterns of cells on a digital sensor array. Through digital processing of the recorded lensfree holographic images, automated counting of red blood cells (RBCs) at densities up to ∼0.4 Million cells/μL is performed, along with characterization of the cell volume histogram and hemoglobin density of RBCs in whole blood. We further demonstrate automated counting of white blood cells using the same platform, also yielding sub-cellular spatial resolution to differentiate granulocytes, monocytes and lymphocytes from each other.
AB - We present a lensfree holographic microscopy platform toward high-throughput on-chip blood analysis, which may facilitate point-of-care diagnostics in resource-poor environments. In this lensfree imaging modality, whole blood samples are illuminated with a spatially incoherent light source to record holographic diffraction patterns of cells on a digital sensor array. Through digital processing of the recorded lensfree holographic images, automated counting of red blood cells (RBCs) at densities up to ∼0.4 Million cells/μL is performed, along with characterization of the cell volume histogram and hemoglobin density of RBCs in whole blood. We further demonstrate automated counting of white blood cells using the same platform, also yielding sub-cellular spatial resolution to differentiate granulocytes, monocytes and lymphocytes from each other.
KW - High-throughput Imaging and Screening
KW - Holographic LUCAS
KW - Lensless / Lensfree Imaging
KW - LUCAS
KW - On-chip Imaging
KW - Point-of-care Diagnostics
KW - Telemedicine Microscopy
KW - Whole Blood Analysis
UR - http://www.scopus.com/inward/record.url?scp=84884387292&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84884387292
SN - 9781618390622
T3 - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
SP - 1424
EP - 1426
BT - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
T2 - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Y2 - 3 October 2010 through 7 October 2010
ER -