TY - GEN
T1 - High-speed three-dimensional fluorescence imaging of neural activity
AU - Holy, Timothy E.
AU - Holekamp, Terrence F.
AU - Turaga, Diwakar
PY - 2006/12/1
Y1 - 2006/12/1
N2 - Our progress in understanding the function of the nervous system is, in many ways, limited by our ability to record neural activity. While there has been considerable progress in imaging at the extremes of length scales (e.g., whole brain imaging by fMRI and sub-cellular resolution by two-photon microscopy), there is a need for optical techniques to record the individual activities of large populations of neurons in intact circuits. Current techniques such as epifluorescence microscopy have reasonable time resolution but suffer from signal-to-background problems when viewing thick samples, while confocal techniques are limited in time resolution and/or photon efficiency. To address some of these limitations, we have developed a novel form of microscopy for high-speed three-dimensional fluorescence imaging. Using this technique, entire stacks of high signal-to-noise images can be acquired in seconds. We are currently using this technique to record the activity of populations of sensory neurons in the mouse olfactory system.
AB - Our progress in understanding the function of the nervous system is, in many ways, limited by our ability to record neural activity. While there has been considerable progress in imaging at the extremes of length scales (e.g., whole brain imaging by fMRI and sub-cellular resolution by two-photon microscopy), there is a need for optical techniques to record the individual activities of large populations of neurons in intact circuits. Current techniques such as epifluorescence microscopy have reasonable time resolution but suffer from signal-to-background problems when viewing thick samples, while confocal techniques are limited in time resolution and/or photon efficiency. To address some of these limitations, we have developed a novel form of microscopy for high-speed three-dimensional fluorescence imaging. Using this technique, entire stacks of high signal-to-noise images can be acquired in seconds. We are currently using this technique to record the activity of populations of sensory neurons in the mouse olfactory system.
UR - http://www.scopus.com/inward/record.url?scp=42749100514&partnerID=8YFLogxK
U2 - 10.1109/LSSA.2006.250367
DO - 10.1109/LSSA.2006.250367
M3 - Conference contribution
AN - SCOPUS:42749100514
SN - 1424402786
SN - 9781424402786
T3 - 2006 IEEE/NLM Life Science Systems and Applications Workshop, LiSA 2006
BT - 2006 IEEE/NLM Life Science Systems and Applications Workshop, LiSA 2006
T2 - 2006 IEEE/NLM Life Science Systems and Applications Workshop, LiSA 2006
Y2 - 13 July 2006 through 14 July 2006
ER -