High-resolution typing of Toxoplasma gondii using microsatellite loci

C. R. Blackston, J. P. Dubey, E. Dotson, C. Su, P. Thulliez, D. Sibley, T. Lehmann

Research output: Contribution to journalArticlepeer-review

44 Scopus citations

Abstract

High-resolution typing of Toxoplasma gondii is essential to understand the effect of genetic differences among strains on the variation in disease manifestation and transmission patterns. Current typing methods discern 3 lineages with minimal within-lineage variation. Described here are 6 new variable loci. These loci, including a minisatellite and 5 microsatellites, were more polymorphic than allozymes, restriction fragment length polymorphisms, and sequence variation in introns. Most importantly, these loci revealed, for the first time, substantial within-lineage variation that was over 6-fold higher than that detected by other markers. Genotyping at these loci facilitates classification of isolates beyond the lineage level.

Original languageEnglish
Pages (from-to)1472-1475
Number of pages4
JournalJournal of Parasitology
Volume87
Issue number6
DOIs
StatePublished - 2001

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