High resolution R banding in amniotic fluid cells using the BrdU-Hoechst 33258-Giemsa (RBG) technique

Sau W. Cheung; James P. Crane; Ann C. Burgess

doi:10.1007/BF00295535

High resolution R banding in amniotic fluid cells using the BrdU-Hoechst 33258-Giemsa (RBG) technique

Sau W. Cheung
, James P. Crane
, Ann C. Burgess

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

While standard Giemsa banding is generally adequate for amniotic fluid chromosome analysis, small deletions, duplications, or translocation breakpoints involving Gnegative bands may be difficult to appreciate. We report a method for producing high resolution R banding in human amniotic fluid cultures using the BrdU-Hoechst 33258-Giemsa (RBG) technique. RBG banding can be useful in confirming and precisely defining structural abnormalities in amniotic fluid cultures.

Original language	English
Pages (from-to)	86-87
Number of pages	2
Journal	Human genetics
Volume	69
Issue number	1
DOIs	https://doi.org/10.1007/BF00295535
State	Published - Jan 1985

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title = "High resolution R banding in amniotic fluid cells using the BrdU-Hoechst 33258-Giemsa (RBG) technique",

abstract = "While standard Giemsa banding is generally adequate for amniotic fluid chromosome analysis, small deletions, duplications, or translocation breakpoints involving Gnegative bands may be difficult to appreciate. We report a method for producing high resolution R banding in human amniotic fluid cultures using the BrdU-Hoechst 33258-Giemsa (RBG) technique. RBG banding can be useful in confirming and precisely defining structural abnormalities in amniotic fluid cultures.",

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year = "1985",

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AU - Burgess, Ann C.

PY - 1985/1

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N2 - While standard Giemsa banding is generally adequate for amniotic fluid chromosome analysis, small deletions, duplications, or translocation breakpoints involving Gnegative bands may be difficult to appreciate. We report a method for producing high resolution R banding in human amniotic fluid cultures using the BrdU-Hoechst 33258-Giemsa (RBG) technique. RBG banding can be useful in confirming and precisely defining structural abnormalities in amniotic fluid cultures.

AB - While standard Giemsa banding is generally adequate for amniotic fluid chromosome analysis, small deletions, duplications, or translocation breakpoints involving Gnegative bands may be difficult to appreciate. We report a method for producing high resolution R banding in human amniotic fluid cultures using the BrdU-Hoechst 33258-Giemsa (RBG) technique. RBG banding can be useful in confirming and precisely defining structural abnormalities in amniotic fluid cultures.

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DO - 10.1007/BF00295535

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High resolution R banding in amniotic fluid cells using the BrdU-Hoechst 33258-Giemsa (RBG) technique

Abstract

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