High resolution R banding in amniotic fluid cells using the BrdU-Hoechst 33258-Giemsa (RBG) technique

Sau W. Cheung, James P. Crane, Ann C. Burgess

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

While standard Giemsa banding is generally adequate for amniotic fluid chromosome analysis, small deletions, duplications, or translocation breakpoints involving Gnegative bands may be difficult to appreciate. We report a method for producing high resolution R banding in human amniotic fluid cultures using the BrdU-Hoechst 33258-Giemsa (RBG) technique. RBG banding can be useful in confirming and precisely defining structural abnormalities in amniotic fluid cultures.

Original languageEnglish
Pages (from-to)86-87
Number of pages2
JournalHuman genetics
Volume69
Issue number1
DOIs
StatePublished - Jan 1 1985

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