High-resolution MRI characterization of human thrombus using a novel fibrin-targeted paramagnetic nanoparticle contrast agent

Xin Yu, Sheng Kwei Song, Junjie Chen, Michael J. Scott, Ralph J. Fuhrhop, Christopher S. Hall, Patrick J. Gaffney, Samuel A. Wickline, Gregory M. Lanza

Research output: Contribution to journalArticlepeer-review

226 Scopus citations

Abstract

In this study, the sensitivity of a novel fibrin-targeted contrast agent for fibrin detection was defined in vitro on human thrombus. The contrast agent was a lipid-encapsulated perfluorocarbon nanoparticle with numerous Gd-DTPA complexes incorporated into the outer surface. After binding to fibrin clots, scanning electron microscopy of treated clots revealed dense accumulation of nanoparticles on the clot surfaces. Fibrin clots with sizes ranging from 0.5-7.0 mm were imaged at 4.7 T with or without treatment with the targeted contrast agent. Regardless of sizes, untreated clots were not detectable by T1-weighted MRI, while targeted contrast agent dramatically improved the detectability of all clots. Decreases in T1 and T2 relaxation times (20-40%) were measured relative to the surrounding media and the control clots. These results suggest the potential for sensitive and specific detection of microthrombi that form on the intimal surfaces of unstable atherosclerotic plaque. (C) 2000 Wiley-Liss, Inc.

Original languageEnglish
Pages (from-to)867-872
Number of pages6
JournalMagnetic resonance in medicine
Volume44
Issue number6
DOIs
StatePublished - 2000

Keywords

  • Fibrin
  • Gadolinium-DTPA
  • MRI
  • Molecular imaging
  • Nanoparticles

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