High-resolution imaging reveals how the spindle midzone impacts chromosome movement

Melissa C. Pamula, Lina Carlini, Scott Forth, Priyanka Verma, Subbulakshmi Suresh, Wesley R. Legant, Alexey Khodjakov, Eric Betzig, Tarun M. Kapoor

Research output: Contribution to journalArticlepeer-review

38 Scopus citations


In the spindle midzone, microtubules from opposite half-spindles form bundles between segregating chromosomes. Microtubule bundles can either push or restrict chromosome movement during anaphase in different cellular contexts, but how these activities are achieved remains poorly understood. Here, we use high-resolution live-cell imaging to analyze individual microtubule bundles, growing filaments, and chromosome movement in dividing human cells. Within bundles, filament overlap length marked by the cross-linking protein PRC1 decreases during anaphase as chromosome segregation slows. Filament ends within microtubule bundles appear capped despite dynamic PRC1 turnover and submicrometer proximity to growing microtubules. Chromosome segregation distance and rate are increased in two human cell lines when microtubule bundle assembly is prevented via PRC1 knockdown. Upon expressing a mutant PRC1 with reduced microtubule affinity, bundles assemble but chromosome hypersegregation is still observed. We propose that microtubule overlap length reduction, typically linked to pushing forces generated within filament bundles, is needed to properly restrict spindle elongation and position chromosomes within daughter cells.

Original languageEnglish
Pages (from-to)2529-2544
Number of pages16
JournalJournal of Cell Biology
Issue number8
StatePublished - Aug 5 2019


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