TY - JOUR
T1 - High prevalence of unusual genotypes of Toxoplasma gondii infection in pork meat samples from Erechim, Southern Brazil
AU - Belfort-Neto, Rubens
AU - Nussenblatt, Veronique
AU - Rizzo, Luiz
AU - Muccioli, Cristina
AU - Silveira, Claudio
AU - Nussenblatt, Robert
AU - Khan, Assis
AU - David Sibley, L.
AU - Belfort, Rubens
PY - 2007/3
Y1 - 2007/3
N2 - Toxoplasmosis is the most common cause of infectious uveitis in Brazil, with a higher frequency in the South of the country. We have collected samples from porcine tongue and diaphragm obtained in both large and small abattoirs and used molecular biological technique to determine the prevalence of infection and RFLP analysis to type the parasites. Seventeen out of 50 (34%) samples from the diaphragm and 33 out of 50 (66%) samples from the tongue demonstrated a positive PCR reaction for T. gondii and restriction analysis of four of the positive samples revealed that all had a type I genotype at SAG2. However, when other unlinked loci were analyzed, these strains had a type III genotype at markers BTUB, SAG3, and GRA6. One of the strains (8T) had a type II allele at SAG3, indicating it has a combination of alleles normally seen in the clonal lineages. Our sampling indicates a high prevalence of infection and suggests that unusual genotypes of T. gondii are found in Brazil even among domesticated pigs.
AB - Toxoplasmosis is the most common cause of infectious uveitis in Brazil, with a higher frequency in the South of the country. We have collected samples from porcine tongue and diaphragm obtained in both large and small abattoirs and used molecular biological technique to determine the prevalence of infection and RFLP analysis to type the parasites. Seventeen out of 50 (34%) samples from the diaphragm and 33 out of 50 (66%) samples from the tongue demonstrated a positive PCR reaction for T. gondii and restriction analysis of four of the positive samples revealed that all had a type I genotype at SAG2. However, when other unlinked loci were analyzed, these strains had a type III genotype at markers BTUB, SAG3, and GRA6. One of the strains (8T) had a type II allele at SAG3, indicating it has a combination of alleles normally seen in the clonal lineages. Our sampling indicates a high prevalence of infection and suggests that unusual genotypes of T. gondii are found in Brazil even among domesticated pigs.
KW - Genotypes
KW - Ocular toxoplasmosis
KW - Toxoplasma gondii
UR - http://www.scopus.com/inward/record.url?scp=34047106355&partnerID=8YFLogxK
U2 - 10.1590/s0001-37652007000100013
DO - 10.1590/s0001-37652007000100013
M3 - Article
C2 - 17401480
AN - SCOPUS:34047106355
SN - 0001-3765
VL - 79
SP - 111
EP - 114
JO - Anais da Academia Brasileira de Ciencias
JF - Anais da Academia Brasileira de Ciencias
IS - 1
ER -