High-molecular-weight aggregates in repackaged bevacizumab

Malik Y. Kahook, Lu Liu, Philip Ruzycki, Naresh Mandava, John F. Carpenter, J. Mark Petrash, David A. Ammar

Research output: Contribution to journalArticlepeer-review

109 Scopus citations


PURPOSE:: The antivascular endothelial growth factor agents ranibizumab and bevaci-zumab are used to treat ocular neovascular diseases. There have been recent reports of sustained elevation of intraocular pressure after use of either agent, which we hypothesize could be because of high-molecular-weight aggregates. METHODS:: Enzyme-linked immunosorbent assay, size exclusion chromatography, and polyacrylamide gel electrophoresis were used to analyze repackaged bevacizumab syringes obtained from three outside compounding pharmacies and samples obtained directly from the original vial. Microflow imaging was used to examine particulate material within samples. RESULTS:: All syringes contained statistically similar amounts of protein, consisting of immunoglobulin (IgG) heavy and light chains (polyacrylamide gel electrophoresis). However, two of the three compounding pharmacies batches had significantly less functional IgG in the solution (enzyme-linked immunosorbent assay). Additionally, the compounding pharmacies with the lowest IgG (∼50%) also contained 10-fold the number of micron-sized particulate matter as measured by microflow imaging. CONCLUSION:: There are significant differences in IgG concentration measured from repackaged bevacizumab syringes. A trend exists for an increase in micron-sized protein aggregates with the decrease in IgG concentration. Large particulate matter within some samples may lead to obstruction of aqueous outflow and subsequent elevation in intraocular pressure. Additional studies are warranted to explore these findings.

Original languageEnglish
Pages (from-to)887-892
Number of pages6
Issue number6
StatePublished - Jun 2010


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