High glucose levels decrease proliferation of cultured human fetal cells from placenta

D. Michael Nelson, Edward M. Curran

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

We used the placenta as a source of undifferentiated cells to study the effect high glucose levels can have on human fetal cell proliferation in vitro. Cells were subcultured in a modified minimum essential medium with 10% fetal bovine serum containing either 5.5 mmol/L (100 mg/dl) d-glucose (control), 11 mmol/L (200 mg/dl) d-glucose, or 22 mmol/L (400 mg/dl) d-glucose. Cells grown in mannitol-containing media were used as controls for osmolality. After 3 and 7 days' growth in different media, the labeling index was determined by autoradiographic analysis, and cell numbers were determined with a Coulter counter. The labeling indices for cells grown 3 days in 11 or 22 mmol/L d-glucose were 89% (p < 0.002) and 84% (p < 0.001), respectively, of control cells grown in 5.5 mmol/L d-glucose. After 7 days' growth, the labeling indices of cells grown in 11 or 22 mmol/L d-glucose were 84% (p < 0.002) and 70% (p < 0.001), respectively, of cells grown in 5.5 mmol/L d-glucose media. There was a significant decrease in the number of cells present at both 3 and 7 days in cultures grown in 22 mmol/L d-glucose compared with control. We conclude that a few day's exposure to high glucose levels can have an effect on proliferation of human placental cells in vitro. We suggest that a glucose effect on proliferation of other cells derived from the products of conception might be one mechanism contributing to abnormal development in some pregnancies of diabetic women.

Original languageEnglish
Pages (from-to)1553-1558
Number of pages6
JournalAmerican journal of obstetrics and gynecology
Volume161
Issue number6 PART 1
DOIs
StatePublished - Dec 1989

Keywords

  • High glucose
  • cell proliferation
  • placenta

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