High-frequency secondary mutations after suicide-driven allelic exchange mutagenesis in extraintestinal pathogenic Escherichia coli

James R. Johnson, Hank A. Lockman, Krista Owens, Srdjan Jelacic, Phillip I. Tarr

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Frequent unintended secondary mutations occurred in extraintestinal pathogenic Escherichia coli strains CP9, CFT073, and RS218 during suicide plasmid-mediated, putatively specific deletions of hlyA, papG allele III, and iha. Pulsed-field gel electrophoresis and PCR analyses demonstrated genomic alterations and/or unintended loss of defined virulence genes (papG, the F7-2 papA allele, iutA, sat, hlyD, and cnf). Caution is warranted when attributing the observed phenotypic changes to the intended mutation.

Original languageEnglish
Pages (from-to)5301-5305
Number of pages5
JournalJournal of bacteriology
Volume185
Issue number17
DOIs
StatePublished - Sep 1 2003

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