High-field 13C NMR spectroscopy of tissue in Vivo. A double-resonance surface-coil probe

Nicholas V. Reo, Coleen S. Ewy, Barry A. Siegfried, Joseph J.H. Ackerman

Research output: Contribution to journalArticle

37 Scopus citations

Abstract

A double-resonance surface-coil NMR probe is described for performance of high-field (8.5 T) proton decoupled carbon-13 experiments with tissue in vivo. The probe may be accommodated in standard, 89 mm i.d. clear bore, commercial spectrometers and is suitable for studies utilizing small laboratory animals such as mice, hamsters, and rats. A coaxial coil design is employed (10 mm diameter 13C coil, 20 mm diameter 1H coil) which provides ca. 40 dB attenuation between the 13C observe and 1H decouple channels. The inherent efficiency of the surface-coil configuration provides a sensitivity comparable to a commercial probe of the same nominal dimension (10 mm Helmholtz coil) and assures adequate decoupling in conductive samples with ca. 3-5 W power. In the absence of 13C isotopic enrichment, NMR spectra of rat leg, liver, and brain in vivo provide signalto-noise sufficient for 10 min time resolution. Administration of 100 mg of 90% 13C-labeled glucose into a peripheral vein of a ca. 300 g rat resulted in a liver glucose resonance which could be monitored with good signal-to-noise and 3 min time resolution.

Original languageEnglish
Pages (from-to)76-84
Number of pages9
JournalJournal of Magnetic Resonance (1969)
Volume58
Issue number1
DOIs
StatePublished - Jun 1 1984

Fingerprint Dive into the research topics of 'High-field <sup>13</sup>C NMR spectroscopy of tissue in Vivo. A double-resonance surface-coil probe'. Together they form a unique fingerprint.

  • Cite this