Abstract
RNA interference methodology suppresses gene expression, thus mimicking loss-of-function mutation and enabling in vitro and in vivo gene function analysis. In this study, we used retroviral and lentiviral vectors to deliver small interfering RNAs and report high-efficiency silencing of a green fluorescent protein (GFP) trans gene and the stem cell-specific transcription factors Oct4/POU5F1 and Nanog in human embryonic stem cells. Gene knockdown of Oct4 and Nanog promotes differentiation, thereby demonstrating a role for these factors in human embryonic stem cell self-renewal.
Original language | English |
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Pages (from-to) | 299-305 |
Number of pages | 7 |
Journal | STEM CELLS |
Volume | 23 |
Issue number | 3 |
DOIs | |
State | Published - Mar 2005 |
Keywords
- Human embryonic stem cells
- Nanog
- Oct4
- RNA interference
- Retroviral and lentiviral vectors
- Self-renewal
- Transgene expression