High-efficiency RNA interference in human embryonic stem cells

Holm Zaehres, M. William Lensch, Laurence Daheron, Sheila A. Stewart, Joseph Itskovitz-Eldor, George Q. Daley

Research output: Contribution to journalArticlepeer-review

225 Scopus citations


RNA interference methodology suppresses gene expression, thus mimicking loss-of-function mutation and enabling in vitro and in vivo gene function analysis. In this study, we used retroviral and lentiviral vectors to deliver small interfering RNAs and report high-efficiency silencing of a green fluorescent protein (GFP) trans gene and the stem cell-specific transcription factors Oct4/POU5F1 and Nanog in human embryonic stem cells. Gene knockdown of Oct4 and Nanog promotes differentiation, thereby demonstrating a role for these factors in human embryonic stem cell self-renewal.

Original languageEnglish
Pages (from-to)299-305
Number of pages7
Issue number3
StatePublished - Mar 2005


  • Human embryonic stem cells
  • Nanog
  • Oct4
  • RNA interference
  • Retroviral and lentiviral vectors
  • Self-renewal
  • Transgene expression


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