High-efficiency RNA interference in human embryonic stem cells

Holm Zaehres, M. William Lensch, Laurence Daheron, Sheila A. Stewart, Joseph Itskovitz-Eldor, George Q. Daley

Research output: Contribution to journalArticle

219 Scopus citations

Abstract

RNA interference methodology suppresses gene expression, thus mimicking loss-of-function mutation and enabling in vitro and in vivo gene function analysis. In this study, we used retroviral and lentiviral vectors to deliver small interfering RNAs and report high-efficiency silencing of a green fluorescent protein (GFP) trans gene and the stem cell-specific transcription factors Oct4/POU5F1 and Nanog in human embryonic stem cells. Gene knockdown of Oct4 and Nanog promotes differentiation, thereby demonstrating a role for these factors in human embryonic stem cell self-renewal.

Original languageEnglish
Pages (from-to)299-305
Number of pages7
JournalSTEM CELLS
Volume23
Issue number3
DOIs
StatePublished - Mar 1 2005

Keywords

  • Human embryonic stem cells
  • Nanog
  • Oct4
  • RNA interference
  • Retroviral and lentiviral vectors
  • Self-renewal
  • Transgene expression

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    Zaehres, H., Lensch, M. W., Daheron, L., Stewart, S. A., Itskovitz-Eldor, J., & Daley, G. Q. (2005). High-efficiency RNA interference in human embryonic stem cells. STEM CELLS, 23(3), 299-305. https://doi.org/10.1634/stemcells.2004-0252