Abstract
RNA interference methodology suppresses gene expression, thus mimicking loss-of-function mutation and enabling in vitro and in vivo gene function analysis. In this study, we used retroviral and lentiviral vectors to deliver small interfering RNAs and report high-efficiency silencing of a green fluorescent protein (GFP) trans gene and the stem cell-specific transcription factors Oct4/POU5F1 and Nanog in human embryonic stem cells. Gene knockdown of Oct4 and Nanog promotes differentiation, thereby demonstrating a role for these factors in human embryonic stem cell self-renewal.
| Original language | English |
|---|---|
| Pages (from-to) | 299-305 |
| Number of pages | 7 |
| Journal | STEM CELLS |
| Volume | 23 |
| Issue number | 3 |
| DOIs | |
| State | Published - Mar 1 2005 |
Keywords
- Human embryonic stem cells
- Nanog
- Oct4
- RNA interference
- Retroviral and lentiviral vectors
- Self-renewal
- Transgene expression
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Cite this
Zaehres, H., Lensch, M. W., Daheron, L., Stewart, S. A., Itskovitz-Eldor, J., & Daley, G. Q. (2005). High-efficiency RNA interference in human embryonic stem cells. STEM CELLS, 23(3), 299-305. https://doi.org/10.1634/stemcells.2004-0252