TY - JOUR
T1 - High-efficiency RNA interference in human embryonic stem cells
AU - Zaehres, Holm
AU - Lensch, M. William
AU - Daheron, Laurence
AU - Stewart, Sheila A.
AU - Itskovitz-Eldor, Joseph
AU - Daley, George Q.
PY - 2005/3
Y1 - 2005/3
N2 - RNA interference methodology suppresses gene expression, thus mimicking loss-of-function mutation and enabling in vitro and in vivo gene function analysis. In this study, we used retroviral and lentiviral vectors to deliver small interfering RNAs and report high-efficiency silencing of a green fluorescent protein (GFP) trans gene and the stem cell-specific transcription factors Oct4/POU5F1 and Nanog in human embryonic stem cells. Gene knockdown of Oct4 and Nanog promotes differentiation, thereby demonstrating a role for these factors in human embryonic stem cell self-renewal.
AB - RNA interference methodology suppresses gene expression, thus mimicking loss-of-function mutation and enabling in vitro and in vivo gene function analysis. In this study, we used retroviral and lentiviral vectors to deliver small interfering RNAs and report high-efficiency silencing of a green fluorescent protein (GFP) trans gene and the stem cell-specific transcription factors Oct4/POU5F1 and Nanog in human embryonic stem cells. Gene knockdown of Oct4 and Nanog promotes differentiation, thereby demonstrating a role for these factors in human embryonic stem cell self-renewal.
KW - Human embryonic stem cells
KW - Nanog
KW - Oct4
KW - RNA interference
KW - Retroviral and lentiviral vectors
KW - Self-renewal
KW - Transgene expression
UR - http://www.scopus.com/inward/record.url?scp=15544388092&partnerID=8YFLogxK
U2 - 10.1634/stemcells.2004-0252
DO - 10.1634/stemcells.2004-0252
M3 - Article
C2 - 15749924
AN - SCOPUS:15544388092
VL - 23
SP - 299
EP - 305
JO - Stem cells (Dayton, Ohio)
JF - Stem cells (Dayton, Ohio)
SN - 1066-5099
IS - 3
ER -