One limit to successful receptor-mediated gene delivery is the exit of the endocytosed material from the endosome. We demonstrate here the delivery of marker genes to tissue culture cells using a modification of the receptor- mediated gene delivery technique that exploits the endosomolytic activity of defective adenovirus particles. In particular, >90% of the transfected-cell population is found to express a β-galactosidase gene, and, most importantly, this high level of expression can be obtained with psoralen- inactivated virus particles. Furthermore, because the delivered gene is not carried within the genome of the adenovirus particle, the size constraints are relieved, and we can, therefore, show the delivery of a 48-kilobase cosmid DNA molecule.
|Number of pages||5|
|Journal||Proceedings of the National Academy of Sciences of the United States of America|
|State||Published - 1992|
- gene therapy