High-density lipoprotein receptor SCARB1 is required for carotenoid coloration in birds

Matthew B. Toomey, Ricardo J. Lopes, Pedro M. Araújo, James D. Johnson, Malgorzata A. Gazda, Sandra Afonso, Paulo G. Mota, Rebecca E. Koch, Geoffrey E. Hill, Joseph C. Corbo, Miguel Carneiro

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30 Scopus citations

Abstract

Yellow, orange, and red coloration is a fundamental aspect of avian diversity and serves as an important signal in mate choice and aggressive interactions. This coloration is often produced through the deposition of diet-derived carotenoid pigments, yet the mechanisms of carotenoid uptake and transport are not wellunderstood. The white recessive breed of the common canary (Serinus canaria), which carries an autosomal recessive mutation that renders its plumage pure white, provides a unique opportunity to investigate mechanisms of carotenoid coloration. We carried out detailed genomic and biochemical analyses comparing the white recessive with yellow and red breeds of canaries. Biochemical analysis revealed that carotenoids are absent or at very low concentrations in feathers and several tissues of white recessive canaries, consistent with a genetic defect in carotenoid uptake. Using a combination of genetic mapping approaches, we show that the white recessive allele is due to a splice donor site mutation in the scavenger receptor B1 (SCARB1; also known as SR-B1) gene. This mutation results in abnormal splicing, with the most abundant transcript lacking exon 4. Through functional assays, we further demonstrate that wild-type SCARB1 promotes cellular uptake of carotenoids but that this function is lost in the predominant mutant isoform in white recessive canaries. Our results indicate that SCARB1 is an essential mediator of the expression of carotenoid-based coloration in birds, and suggest a potential link between visual displays and lipid metabolism.

Original languageEnglish
Pages (from-to)5219-5224
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume114
Issue number20
DOIs
StatePublished - May 16 2017

Keywords

  • Carotenoids
  • Coloration
  • Lipid metabolism
  • Serinus canaria

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    Toomey, M. B., Lopes, R. J., Araújo, P. M., Johnson, J. D., Gazda, M. A., Afonso, S., Mota, P. G., Koch, R. E., Hill, G. E., Corbo, J. C., & Carneiro, M. (2017). High-density lipoprotein receptor SCARB1 is required for carotenoid coloration in birds. Proceedings of the National Academy of Sciences of the United States of America, 114(20), 5219-5224. https://doi.org/10.1073/pnas.1700751114