Hierarchical action and inhibition of plant dicer-like proteins in antiviral defense

Angélique Deleris, Javier Gallago-Bartolome, Jinsong Bao, Kristin D. Kasschau, James C. Carrington, Olivier Voinnet

Research output: Contribution to journalArticlepeer-review

679 Scopus citations


The mechanisms underlying induction and suppression of RNA silencing in the ongoing plant-virus arms race are poorly understood. We show here that virus-derived small RNAs produced by Arabidopsis Dicer-like 4 (DCL4) program an effector complex conferring antiviral immunity. Inhibition of DCL4 by a viral-encoded suppressor revealed the subordinate antiviral activity of DCL2. Accordingly, inactivating both DCL2 and DCL4 was necessary and sufficient to restore systemic infection of a suppressor-deficient virus. The effects of DCL2 were overcome by increasing viral dosage in inoculated leaves, but this could not surmount additional, non-cell autonomous effects of DCL4 specifically preventing viral unloading from the vasculature. These findings define a molecular framework for studying antiviral silencing and defense in plants.

Original languageEnglish
Pages (from-to)68-71
Number of pages4
Issue number5783
StatePublished - Jul 7 2006


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