TY - JOUR
T1 - Heterogeneity of p15(E)-related polypeptides expressed by MuLV-infected cells
AU - Bach, Richard G.
AU - Meruelo, Daniel
N1 - Funding Information:
We thank Dr. B. Chesebro, Dr. .I, W. Hartley, Dr. A. Mayer, and Dr. T. Pincus for their kind gifts of cells, viruses, and antibody. This work was supported by Grants CA31346 and CA22247 from the National Institutes of Health, Grant 5T32GM07308 from the National Institutes of General Medical Sciences, and grants from the American Cancer Society and the Irma T. Hirsch1 Foundation. Richard G. Bach is a fellow of the Medical Scientist Training Program; Daniel Meruelo is a Leukemia Society of America Scholar.
PY - 1985/8
Y1 - 1985/8
N2 - The p15(E)-related polypeptides of radiation leukemia virus (RadLV)-derived viruses and of cells infected with prototype MuLV were analyzed by immunoprecipitation, SDS-PAGE, and immunofluorescence analyses. It was found that the p15(E)-related molecules of ecotropic and xenotropic viruses derived from RadLV lymphoma cell lines were distinguishable by reactivity with monoclonal anti-p15(E) antibodies and by SDS-PAGE profile. Ecotropic MuLV of RadLV origin encoded the p15(E)a antigen and produced a Pr15(E) of 20K MW. In contrast, xenotropic virus derived from RadLV did not express the p15(E)a antigen and by SDS-PAGE its Pr15(E) migrated at 21K. A previously undescribed, p15(E)-related molecule of 18.5K MW was associated with xenotropic RadLV. These differences were also reproduced by the prototype ecotropic, xenotropic, and dualtropic viruses.
AB - The p15(E)-related polypeptides of radiation leukemia virus (RadLV)-derived viruses and of cells infected with prototype MuLV were analyzed by immunoprecipitation, SDS-PAGE, and immunofluorescence analyses. It was found that the p15(E)-related molecules of ecotropic and xenotropic viruses derived from RadLV lymphoma cell lines were distinguishable by reactivity with monoclonal anti-p15(E) antibodies and by SDS-PAGE profile. Ecotropic MuLV of RadLV origin encoded the p15(E)a antigen and produced a Pr15(E) of 20K MW. In contrast, xenotropic virus derived from RadLV did not express the p15(E)a antigen and by SDS-PAGE its Pr15(E) migrated at 21K. A previously undescribed, p15(E)-related molecule of 18.5K MW was associated with xenotropic RadLV. These differences were also reproduced by the prototype ecotropic, xenotropic, and dualtropic viruses.
UR - http://www.scopus.com/inward/record.url?scp=0022391717&partnerID=8YFLogxK
U2 - 10.1016/0042-6822(85)90211-9
DO - 10.1016/0042-6822(85)90211-9
M3 - Article
C2 - 2409668
AN - SCOPUS:0022391717
VL - 145
SP - 165
EP - 170
JO - Virology
JF - Virology
SN - 0042-6822
IS - 1
ER -