Background The concept that pore-forming Kir6.2 and regulatory SUR2A subunits form cardiac ATP-sensitive potassium (KATP) channels is challenged by recent reports that SUR1 is predominant in mouse atrial K ATP channels. Objective To assess SUR subunit composition of K ATP channels and consequence of KATP activation for action potential duration (APD) in dog hearts. Methods Patch-clamp techniques were used on isolated dog cardiomyocytes to investigate KATP channel properties. Dynamic current clamp, by injection of a linear K+ conductance to simulate activation of the native current, was used to study the consequences of KATP activation on APD. Results Metabolic inhibitor (MI)-activated current was not significantly different from pinacidil (SUR2A-specific)-activated current, and both currents were larger than diazoxide (SUR1-specific)-activated current in both the atrium and the ventricle. Mean KATP conductance (activated by MI) did not differ significantly between chambers, although, within the ventricle, both MI-induced and pinacidil-induced currents tended to decrease from the epicardium to the endocardium. Dynamic current-clamp results indicate that myocytes with longer baseline APDs are more susceptible to injected KATP current, a result reproduced in silico by using a canine action potential model (Hund-Rudy) to simulate epicardial and endocardial myocytes. Conclusions Even a small fraction of KATP activation significantly shortens APD in a manner that depends on existing heterogeneity in KATP current and APD.

Original languageEnglish
Pages (from-to)1576-1583
Number of pages8
JournalHeart rhythm
Issue number10
StatePublished - Oct 2013


  • ATP-sensitivepotassiumchannel
  • Actionpotentialduration
  • Canine
  • Diazoxide
  • Metabolicinhibition
  • Modelsimulation
  • Myocyte
  • Patch-clamptechnique
  • Pinacidil
  • Sulfonylureareceptor


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