Hepatitis B virus x gene and cyanobacterial toxins promote aflatoxin B1-induced hepatotumorigenesis in mice

Min Lian, Ying Liu, Shun Zhang Yu, Geng Sun Qian, Shu Guang Wan, Kenneth R. Dixon

Research output: Contribution to journalArticlepeer-review

36 Scopus citations


Aim: To assess the combinative role of aflatoxin B1 (AFB1), cyanobacterial toxins (cyanotoxins), and hepatitis B virus (HBV) × gene in hepatotumorigenicity. Methods: One-week-old animals carrying HBV × gene and their wild-type littermates were intraperitoneally (ip) injected with either single-dose AFB1 [6 mg/kg body weight (bw)], repeated-dose cyanotoxins (microcystinLR or nodularin, 10 μg/kg bw once a week for 15 wk), DMSO (vehicle control) alone, or AFB1, followed. by cyanotoxins a week later, and were sacrificed at 24 and 52 wk post-treatment. Results: AFB 1 induced liver tumors in 13 of 29 (44.8%) transgenic mice at 52 wk post-treatment, significantly more frequent than in wild-type mice (13.3%). This significant difference was not shown in the 24-wk study. Compared with AFB1 exposure alone, MC-LR and nodularin yielded approximately 3-fold and 6-fold increases in the incidence of AFB1-induced liver tumors in wild-type animals at 24 wk, respectively. HBV × gene did not further elevate the risk associated with co-exposure to AFB1 and cyanotoxins. With the exception of an MC-LR-dosed wild-type mouse, no liver tumor was observed in mice treated with cyanotoxins alone at 24 wk. Neither DMSO-treated transgenic mice nor their wild-type littermates had pathologic alterations relevant to hepatotumorigenesis in even up to 52 wk. Conclusion: HBV × gene and nodularin promote the development of AFB1-induced liver tumors. Co-exposure to AFB1 and MC-LR tends to elevate the risk of liver tumors at 24 wk relative to exposure to one of them. The combinative effect of AM, cyanotoxins and HBVx on hepatotumorigenesis is weak at 24 wk.

Original languageEnglish
Pages (from-to)3065-3072
Number of pages8
JournalWorld Journal of Gastroenterology
Issue number18
StatePublished - May 14 2006


  • Aflatoxins
  • Cyanobacteria
  • Hepatitis B virus
  • Liver neoplasms
  • Transgenic mice


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