TY - JOUR
T1 - Hepatic uptake of proteins coupled to fetuin glycopeptide
AU - Rogers, John C.
AU - Kornfeld, Stuart
N1 - Funding Information:
*This work was supported in part by Grants HE-00022 and ROl CA-08759 from the United States Public Health Service and Grant PRA-31 from the American Cancer Society.
PY - 1971/11/5
Y1 - 1971/11/5
N2 - The glycopeptides of fetuin and γ G immunoglobulin have been covalently linked to lysozyme and albumin with the coupling reagent toluene 2,4 diisocyanate. When the fetuin glycopeptide-protein compounds were treated with neuraminidase to remove sialic acid, and then injected into rats, the coupled compounds were rapidly and selectively removed from the circulation by the liver, most likely by the hepatocytes. Removal of the exposed galactose residues of the asialofetuin-proteins with β-galactosidase prevented this clearance. Neither the native proteins nor the γ G glycopeptide-proteins are taken up by the liver, demonstrating the specificity of the phenomenon. Once the asialofetuin-protein complexes enter the hepatocytes, they are degraded within two hours.
AB - The glycopeptides of fetuin and γ G immunoglobulin have been covalently linked to lysozyme and albumin with the coupling reagent toluene 2,4 diisocyanate. When the fetuin glycopeptide-protein compounds were treated with neuraminidase to remove sialic acid, and then injected into rats, the coupled compounds were rapidly and selectively removed from the circulation by the liver, most likely by the hepatocytes. Removal of the exposed galactose residues of the asialofetuin-proteins with β-galactosidase prevented this clearance. Neither the native proteins nor the γ G glycopeptide-proteins are taken up by the liver, demonstrating the specificity of the phenomenon. Once the asialofetuin-protein complexes enter the hepatocytes, they are degraded within two hours.
UR - http://www.scopus.com/inward/record.url?scp=0015212069&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(71)90462-1
DO - 10.1016/0006-291X(71)90462-1
M3 - Article
C2 - 5128172
AN - SCOPUS:0015212069
SN - 0006-291X
VL - 45
SP - 622
EP - 629
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -