TY - JOUR
T1 - Hemodilution reduces early reperfusion injury in an ex vivo rabbit lung preservation model
AU - Puskas, John D.
AU - Oka, Tadayuki
AU - Mayer, Eckhard
AU - Wisser, Wilfried
AU - Dcwney, Gregory P.
AU - Slutsky, Arthur S.
AU - Patterson, G. Alexander
N1 - Funding Information:
We are grateful to acknowledge the superb technical assistance of Joan Mates and Stephanie Diamant. Expert statistical analysis was performed by Dr Kleber N. DeCampos. This work was supported by Medical Research Council of Canada grant 10142. Doctor Puskas was the recipient of the E.D. Churchill Surgical Fellowship from the Massachusetts General Hospital, Harvard Medical School, Boston, MA. We also thank Ethicon Canada Ltd for supplying suture material and Upjohn Canada for their generously providing prostaglandin E, for this study.
PY - 1994/3
Y1 - 1994/3
N2 - We sought to reduce early ischemia-reperfusion injury after lung preservation by an initial brief period of hemodilute reperfusion. Left lungs of New Zealand White rabbits were ventilated with room air and reperfused in an ex vivo ventilation-perfusion apparatus after 18 hours of storage at 10 °C. Lungs were randomly assigned to one of three groups (n = 6) according to the composition of initial reperfusate. In group 1 (control), preserved lungs were reperfused with whole blood for 20 minutes (hematocrit, 38%). In the experimental groups, blood was diluted to a hematocrit of 10% with Ringer's lactate (group 2) or low-potassium-dextran solution (group 3) for the first 10 minutes of reperfusion, followed immediately by whole blood for 10 minutes. Oxygen tension of left ventricular effluent at the end of the 20-minute assessment period was significantly higher in both hemodiluted groups (mean ± standard error of the mean: group 2, 81.3 ± 6.6 mm Hg; group 3, 77.0 ± 9.5 mm Hg, versus Group 1, 46.3 ± 7.4 mm Hg; p < 0.006). Similarly, mean tracheal airway pressure was reduced in the hemodiluted groups, suggesting improved compliance (group 2; 3.1 ± 0.3 mm Hg; group 3, 2.8 ± 0.6 mm Hg; versus group 1, 6.5 ± 1.4 mm Hg; p < 0.05). An initial 10-minute period of hemodilute reperfusion appears to reduce early pulmonary ischemiareperfusion injury in this 18-hour ex vivo rabbit lung preservation model.
AB - We sought to reduce early ischemia-reperfusion injury after lung preservation by an initial brief period of hemodilute reperfusion. Left lungs of New Zealand White rabbits were ventilated with room air and reperfused in an ex vivo ventilation-perfusion apparatus after 18 hours of storage at 10 °C. Lungs were randomly assigned to one of three groups (n = 6) according to the composition of initial reperfusate. In group 1 (control), preserved lungs were reperfused with whole blood for 20 minutes (hematocrit, 38%). In the experimental groups, blood was diluted to a hematocrit of 10% with Ringer's lactate (group 2) or low-potassium-dextran solution (group 3) for the first 10 minutes of reperfusion, followed immediately by whole blood for 10 minutes. Oxygen tension of left ventricular effluent at the end of the 20-minute assessment period was significantly higher in both hemodiluted groups (mean ± standard error of the mean: group 2, 81.3 ± 6.6 mm Hg; group 3, 77.0 ± 9.5 mm Hg, versus Group 1, 46.3 ± 7.4 mm Hg; p < 0.006). Similarly, mean tracheal airway pressure was reduced in the hemodiluted groups, suggesting improved compliance (group 2; 3.1 ± 0.3 mm Hg; group 3, 2.8 ± 0.6 mm Hg; versus group 1, 6.5 ± 1.4 mm Hg; p < 0.05). An initial 10-minute period of hemodilute reperfusion appears to reduce early pulmonary ischemiareperfusion injury in this 18-hour ex vivo rabbit lung preservation model.
UR - http://www.scopus.com/inward/record.url?scp=0028297065&partnerID=8YFLogxK
U2 - 10.1016/0003-4975(94)90577-0
DO - 10.1016/0003-4975(94)90577-0
M3 - Article
C2 - 8147648
AN - SCOPUS:0028297065
SN - 0003-4975
VL - 57
SP - 731
EP - 735
JO - The Annals of thoracic surgery
JF - The Annals of thoracic surgery
IS - 3
ER -