Heme Trafficking and Modifications during System i Cytochrome c Biogenesis: Insights from Heme Redox Potentials of Ccm Proteins

Cytochromes c require covalent attachment of heme via two thioether bonds at conserved CXXCH motifs, a process accomplished in prokaryotes by eight integral membrane proteins (CcmABCDEFGH), termed System I. Heme is trafficked from inside the cell to outside (via CcmABCD) and chaperoned (holoCcmE) to the cytochrome c synthetase (CcmF/H). Purification of key System I pathway intermediates allowed the determination of heme redox potentials. The data support a model whereby heme is oxidized to form holoCcmE and subsequently reduced by CcmF/H for thioether formation, with Fe²⁺ being required for attachment to CXXCH. Results provide insight into mechanisms for the oxidation and reduction of heme in vivo.