@article{3066387d47874f179dcafee7a2e2e59a,
title = "Helium Ion Microscopy (HIM) for the imaging of biological samples at sub-nanometer resolution",
abstract = "Scanning Electron Microscopy (SEM) has long been the standard in imaging the sub-micrometer surface ultrastructure of both hard and soft materials. In the case of biological samples, it has provided great insights into their physical architecture. However, three of the fundamental challenges in the SEM imaging of soft materials are that of limited imaging resolution at high magnification, charging caused by the insulating properties of most biological samples and the loss of subtle surface features by heavy metal coating. These challenges have recently been overcome with the development of the Helium Ion Microscope (HIM), which boasts advances in charge reduction, minimized sample damage, high surface contrast without the need for metal coating, increased depth of field, and 5 angstrom imaging resolution. We demonstrate the advantages of HIM for imaging biological surfaces as well as compare and contrast the effects of sample preparation techniques and their consequences on sub-nanometer ultrastructure.",
author = "Joens, {Matthew S.} and Chuong Huynh and Kasuboski, {James M.} and David Ferranti and Sigal, {Yury J.} and Fabian Zeitvogel and Martin Obst and Burkhardt, {Claus J.} and Curran, {Kevin P.} and Chalasani, {Sreekanth H.} and Stern, {Lewis A.} and Bernhard Goetze and Fitzpatrick, {James A.J.}",
note = "Funding Information: We gratefully acknowledge financial support from the Waitt Advanced Biophotonics Center endowment (J.M.K., M.S.J. and J.A.J.F.), NCI P30 Cancer Center Support Grant CA014195-39 (Y.J.S. and J.A.J.F.), NINDS P30 Neuroscience Center Core Grant NS072031-01A1 (M.S.J. and J.A.J.F.) and DFG Emmy Noether grant OB 362/1-1 (F.Z. and M.O.). We would also like to thank the Ion Microscopy Innovation Center at Carl Zeiss Microscopy (Peabody, MA) for providing access to an Orion PLUS helium ion microscope, Carl Procko from the Chory lab at the Salk Institute for providing wild-type Arabidopsis Thaliana for HIM imaging experiments and Kat Kearny for critical reading of the manuscript and for helpful suggestions regarding organization and formatting.",
year = "2013",
month = dec,
day = "17",
doi = "10.1038/srep03514",
language = "English",
volume = "3",
journal = "Scientific Reports",
issn = "2045-2322",
}