TY - JOUR
T1 - Helicobacter pylori populations in Peruvian patients
AU - Berg, Douglas E.
AU - Gilman, Robert H.
AU - Lelwala-Guruge, Janaki
AU - Srivastava, Kalpana
AU - Valdez, Yanet
AU - Watanabe, Jose
AU - Miyagi, Juan
AU - Akopyants, Natalia S.
AU - Ramirez-Ramos, Alberto
AU - Yoshiwara, Tacano H.
AU - Recavarren, Sixto
AU - Leon-Barua, Raul
N1 - Funding Information:
Grant support: This work was supported by the National Institutes of Health (AI38166, DK48029, and U01AI3584), the Fogarty Center (TW00611), and the American Cancer Society (VM-121). * Present affiliation: Department of Molecular Biology and Pharmacology, Washington University Medical School, St. Louis, Missouri.
PY - 1997
Y1 - 1997
N2 - Helicobacter pylori is an extremely diverse species. The characterization of strains isolated from individual patients should give insights into colonization and disease mechanisms and bacterial evolution. We studied H. pylori isolates from patients in the Japanese-Peruvian Polyclinic in Lima, Peru, by determining metronidazole susceptibility or resistance and by random amplified polymorphic DNA (RAPD) fingerprinting (a measure of overall genotype). Strains isolated from several biopsy specimens from each of 24 patients were studied. Both metronidazole-susceptible and -resistant strains were isolated from 13 patients, whereas strains of more than one RAPD type were isolated from only seven patients. We propose that the homogeneity in RAPD fingerprints for strains isolated from most persons reflects selection for particular H. pylori genotypes during chronic infection in individual hosts and the human diversity in traits that are important to this pathogen. Carriage of related metronidazole-resistant and -susceptible strains could reflect frequent metronidazole use in Peru and alternating selection for resistant and susceptible phenotypes during and after metronidazole therapy.
AB - Helicobacter pylori is an extremely diverse species. The characterization of strains isolated from individual patients should give insights into colonization and disease mechanisms and bacterial evolution. We studied H. pylori isolates from patients in the Japanese-Peruvian Polyclinic in Lima, Peru, by determining metronidazole susceptibility or resistance and by random amplified polymorphic DNA (RAPD) fingerprinting (a measure of overall genotype). Strains isolated from several biopsy specimens from each of 24 patients were studied. Both metronidazole-susceptible and -resistant strains were isolated from 13 patients, whereas strains of more than one RAPD type were isolated from only seven patients. We propose that the homogeneity in RAPD fingerprints for strains isolated from most persons reflects selection for particular H. pylori genotypes during chronic infection in individual hosts and the human diversity in traits that are important to this pathogen. Carriage of related metronidazole-resistant and -susceptible strains could reflect frequent metronidazole use in Peru and alternating selection for resistant and susceptible phenotypes during and after metronidazole therapy.
UR - http://www.scopus.com/inward/record.url?scp=0030831491&partnerID=8YFLogxK
U2 - 10.1086/516081
DO - 10.1086/516081
M3 - Article
C2 - 9402344
AN - SCOPUS:0030831491
SN - 1058-4838
VL - 25
SP - 996
EP - 1002
JO - Clinical Infectious Diseases
JF - Clinical Infectious Diseases
IS - 5
ER -