@inbook{ca42c56229a047a8bb14e4ae82541494,
title = "Heavy methyl-SILAC labeling coupled with liquid chromatography and high-resolution mass spectrometry to study the dynamics of site-specific histone methylation",
abstract = "Histone lysine and arginine methylation involved in gene activation and silencing is dynamically regulated. However, partly limited to the research technologies previously available, the dynamics of global histone methylation on a site-specific basis have not been fully pursued. Heavy methyl-SILAC (Stable Isotope Labeling of Amino Acids in Cell Culture) labeling provides a remarkable signpost to distinguish the preexisting and newly generated methyl marks on histones. Using this technology coupled with quantitative LC-MS analysis make it possible to monitor changes in the dynamics of histone site-specific methylation. In this chapter, we comprehensively describe the experimental strategy to determine the dynamics of multiple histone methylated residues including SILAC labeling, histone extraction/purification and mass spectrometry analysis.",
keywords = "Dynamics, Heavy methionine, Histone, Mass spectrometry, Methylation, Quantitation, SILAC",
author = "Cao, {Xing Jun} and Zee, {Barry M.} and Garcia, {Benjamin A.}",
year = "2013",
doi = "10.1007/978-1-62703-284-1_24",
language = "English",
isbn = "9781627032834",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "299--313",
booktitle = "Gene Regulation",
}