Heat of protonation of endoglucanase V catalytic domain (EGV-core) from Humicola insolens

Roberto Galletto; Francesco Attanasio; Eleftheria Dossi; Martin Schülein; Giovanni Rialdi

doi:10.1016/s0040-6031(98)00434-1

Heat of protonation of endoglucanase V catalytic domain (EGV-core) from Humicola insolens

Roberto Galletto, Francesco Attanasio, Eleftheria Dossi, Martin Schülein, Giovanni Rialdi

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Endoglucanase V catalytic core is a single chain protein of 213 amino acids. The titration of the ionizable groups of the macromolecule has been performed by an isothermal calorimetric technique and the pK⁰_i and ΔH⁰_i related to the groups have been estimated and no apparent abnormality was observed in the titration behavior. These values are in agreement with the expected theoretical ones.

Original language	English
Pages (from-to)	17-21
Number of pages	5
Journal	Thermochimica Acta
Volume	321
Issue number	1-2
DOIs	https://doi.org/10.1016/s0040-6031(98)00434-1
State	Published - Nov 2 1998

Keywords

Cellulase
Heat of protonation
Protein calorimetry
Protein titration

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10.1016/s0040-6031(98)00434-1

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title = "Heat of protonation of endoglucanase V catalytic domain (EGV-core) from Humicola insolens",

abstract = "Endoglucanase V catalytic core is a single chain protein of 213 amino acids. The titration of the ionizable groups of the macromolecule has been performed by an isothermal calorimetric technique and the pK0i and ΔH0i related to the groups have been estimated and no apparent abnormality was observed in the titration behavior. These values are in agreement with the expected theoretical ones.",

keywords = "Cellulase, Heat of protonation, Protein calorimetry, Protein titration",

author = "Roberto Galletto and Francesco Attanasio and Eleftheria Dossi and Martin Sch{\"u}lein and Giovanni Rialdi",

note = "Funding Information: We thank Dr. G. Davies for the CA co-ordinates of catalytic core of Endoglucanase V and Dr. D. Otzen for helpful comments.This work was funded by the European Union (Contract BIO2-CT94-3018)",

year = "1998",

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doi = "10.1016/s0040-6031(98)00434-1",

language = "English",

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journal = "Thermochimica Acta",

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T1 - Heat of protonation of endoglucanase V catalytic domain (EGV-core) from Humicola insolens

AU - Galletto, Roberto

AU - Attanasio, Francesco

AU - Dossi, Eleftheria

AU - Schülein, Martin

AU - Rialdi, Giovanni

N1 - Funding Information: We thank Dr. G. Davies for the CA co-ordinates of catalytic core of Endoglucanase V and Dr. D. Otzen for helpful comments.This work was funded by the European Union (Contract BIO2-CT94-3018)

PY - 1998/11/2

Y1 - 1998/11/2

N2 - Endoglucanase V catalytic core is a single chain protein of 213 amino acids. The titration of the ionizable groups of the macromolecule has been performed by an isothermal calorimetric technique and the pK0i and ΔH0i related to the groups have been estimated and no apparent abnormality was observed in the titration behavior. These values are in agreement with the expected theoretical ones.

AB - Endoglucanase V catalytic core is a single chain protein of 213 amino acids. The titration of the ionizable groups of the macromolecule has been performed by an isothermal calorimetric technique and the pK0i and ΔH0i related to the groups have been estimated and no apparent abnormality was observed in the titration behavior. These values are in agreement with the expected theoretical ones.

KW - Cellulase

KW - Heat of protonation

KW - Protein calorimetry

KW - Protein titration

UR - http://www.scopus.com/inward/record.url?scp=0006533958&partnerID=8YFLogxK

U2 - 10.1016/s0040-6031(98)00434-1

DO - 10.1016/s0040-6031(98)00434-1

M3 - Article

AN - SCOPUS:0006533958

SN - 0040-6031

VL - 321

SP - 17

EP - 21

JO - Thermochimica Acta

JF - Thermochimica Acta

IS - 1-2

ER -

Heat of protonation of endoglucanase V catalytic domain (EGV-core) from Humicola insolens

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