HDAC inhibition results in widespread alteration of the histone acetylation landscape and BRD4 targeting to gene bodies

Mariesa J. Slaughter; Erin K. Shanle; Abid Khan; Katrin F. Chua; Tao Hong; Lisa D. Boxer; C. David Allis; Steven Z. Josefowicz; Benjamin A. Garcia; Scott B. Rothbart; Brian D. Strahl; Ian J. Davis

doi:10.1016/j.celrep.2020.108638

HDAC inhibition results in widespread alteration of the histone acetylation landscape and BRD4 targeting to gene bodies

Mariesa J. Slaughter, Erin K. Shanle, Abid Khan, Katrin F. Chua, Tao Hong, Lisa D. Boxer, C. David Allis, Steven Z. Josefowicz, Benjamin A. Garcia, Scott B. Rothbart, Brian D. Strahl, Ian J. Davis

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Abstract

Slaughter et al. use proteomic and genomic approaches to quantitatively assess the impact of histone deacetylase inhibitor (HDACi) treatment on histone acetylation and BRD4 binding genome-wide. Their studies show that HDACi treatment causes histone polyacetylation and recruitment of BRD4, particularly in the gene bodies of actively transcribed genes.

Original language	English
Article number	108638
Journal	Cell Reports
Volume	34
Issue number	3
DOIs	https://doi.org/10.1016/j.celrep.2020.108638
State	Published - Jan 19 2021

Keywords

BRD4
HDAC
bromodomain
chromatin immunoprecipitation
histone acetylation
mass spectrometry
peptide microarray
superenhancer
transcription

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10.1016/j.celrep.2020.108638

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Slaughter, M. J., Shanle, E. K., Khan, A., Chua, K. F., Hong, T., Boxer, L. D., Allis, C. D., Josefowicz, S. Z., Garcia, B. A., Rothbart, S. B., Strahl, B. D., & Davis, I. J. (2021). HDAC inhibition results in widespread alteration of the histone acetylation landscape and BRD4 targeting to gene bodies. Cell Reports, 34(3), Article 108638. https://doi.org/10.1016/j.celrep.2020.108638

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abstract = "Slaughter et al. use proteomic and genomic approaches to quantitatively assess the impact of histone deacetylase inhibitor (HDACi) treatment on histone acetylation and BRD4 binding genome-wide. Their studies show that HDACi treatment causes histone polyacetylation and recruitment of BRD4, particularly in the gene bodies of actively transcribed genes.",

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AU - Hong, Tao

AU - Boxer, Lisa D.

AU - Allis, C. David

AU - Josefowicz, Steven Z.

AU - Garcia, Benjamin A.

AU - Rothbart, Scott B.

AU - Strahl, Brian D.

AU - Davis, Ian J.

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KW - HDAC

KW - bromodomain

KW - chromatin immunoprecipitation

KW - histone acetylation

KW - mass spectrometry

KW - peptide microarray

KW - superenhancer

KW - transcription

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JO - Cell Reports

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HDAC inhibition results in widespread alteration of the histone acetylation landscape and BRD4 targeting to gene bodies

Abstract

Keywords

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