@article{cb9365eb654f46c7b1f08bb22fa4d4e2,
title = "Hda9-pwr-hos15 is a core histone deacetylase complex regulating transcription and development",
abstract = "Histone deacetylases remove acetyl groups from histone proteins and play important roles in many genomic processes. How histone deacetylases perform specialized molecular and biological functions in plants is poorly understood. Here, we identify HIGH EXPRESSION OF OSMOTICALLY RESPONSIVE GENES 15 (HOS15) as a core member of the Arabidopsis (Arabidopsis thaliana) HISTONE DEACETYLASE9-POWERDRESS (HDA9-PWR) complex. HOS15 immunoprecipitates with both HDA9 and PWR. Mutation of HOS15 induces histone hyperacetylation and methylation changes similar to hda9 and pwr mutants. HOS15, HDA9, and PWR are coexpressed in all organs, and mutant combinations display remarkable phenotypic resemblance and nonadditivity for organogenesis and developmental phase transitions. Ninety percent of HOS15-regulated genes are also controlled by HDA9 and PWR. HDA9 binds to and directly represses 92 genes, many of which are responsive to biotic and abiotic stimuli, including a family of ethylene response factor genes. Additionally, HOS15 regulates HDA9 nuclear accumulation and chromatin association. Collectively, this study establishes that HOS15 forms a core complex with HDA9 and PWR to control gene expression and plant development.",
author = "Mayer, {Kevin S.} and Xiangsong Chen and Dean Sanders and Jiani Chen and Jianjun Jiang and Phu Nguyen and Mark Scalf and Smith, {Lloyd M.} and Xuehua Zhong",
note = "Funding Information: Work in X.Z.{\textquoteright}s laboratory was supported by the NSF CAREER award (MCB-1552455), the USDA (Hatch 1012915), and NIH-NIGMS (R35GM124806). K.S.M. was supported by an NIH Genetics Predoctoral Research Training Grant. Work in L.M.S.{\textquoteright}s laboratory was supported by NIH-NIGMS (R01GM114292). Funding Information: 1Work in X.Z.{\textquoteright}s laboratory was supported by the NSF CAREER award (MCB-1552455), the USDA (Hatch 1012915), and NIH-NIGMS (R35GM124806). K.S.M. was supported by an NIH Genetics Predoctoral Research Training Grant. Work in L.M.S.{\textquoteright}s laboratory was supported by NIH-NIGMS (R01GM114292). 2These authors contributed equally to the article. 3Author for contact: xuehua.zhong@wisc.edu. 4Senior author. The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Xuehua Zhong (xuehua.zhong@wisc.edu). X.Z. designed the study; K.S.M., X.C., J.C., J.J., and P.N. performed experiments; D.S. performed RNA-seq and histone mass spectrometry analysis; M.S. and L.M.S. performed mass spectrometry analysis; K.S.M. and X.C. analyzed all other data; K.S.M., X.C., and X.Z. wrote the manuscript. [OPEN]Articles can be viewed without a subscription. www.plantphysiol.org/cgi/doi/10.1104/pp.18.01156 Publisher Copyright: {\textcopyright} 2019, American Society of Plant Biologists. All rights reserved.",
year = "2019",
month = may,
doi = "10.1104/pp.18.01156",
language = "English",
volume = "180",
pages = "342--355",
journal = "Plant Physiology",
issn = "0032-0889",
number = "1",
}