HCoDES Reveals Chromosomal DNA End Structures with Single-Nucleotide Resolution

Yair Dorsett, Yanjiao Zhou, Anthony T. Tubbs, Bo Ruei Chen, Caitlin Purman, Baeck Seung Lee, Rosmy George, Andrea L. Bredemeyer, Jiang yang Zhao, Erica Sodergen, George M. Weinstock, Nathan D. Han, Alejandro Reyes, Eugene M. Oltz, Dale Dorsett, Ziva Misulovin, Jacqueline E. Payton, Barry P. Sleckman

Research output: Contribution to journalArticlepeer-review

28 Scopus citations


The structure of broken DNA ends is a critical determinant of the pathway used for DNA double-strand break (DSB) repair. Here, we develop an approach involving the hairpin capture of DNA end structures (HCoDES), which elucidates chromosomal DNA end structures at single-nucleotide resolution. HCoDES defines structures of physiologic DSBs generated by the RAG endonuclease, as well as those generated by nucleases widely used for genome editing. Analysis of G1 phase cells deficient in H2AX or 53BP1 reveals DNA ends that are frequently resected to form long single-stranded overhangs that can be repaired by mutagenic pathways. In addition to 3' overhangs, many of these DNA ends unexpectedly form long 5' single-stranded overhangs. The divergence in DNA end structures resolved by HCoDES suggests that H2AX and 53BP1 may have distinct activities in end protection. Thus, the high-resolution end structures obtained by HCoDES identify features of DNA end processing during DSB repair.

Original languageEnglish
Pages (from-to)808-818
Number of pages11
JournalMolecular cell
Issue number6
StatePublished - Dec 18 2014


Dive into the research topics of 'HCoDES Reveals Chromosomal DNA End Structures with Single-Nucleotide Resolution'. Together they form a unique fingerprint.

Cite this