HC-Pro protein of Potato virus Y can interact with three Arabidopsis 20S proteasome subunits in planta

Yongsheng Jin, Dongyuan Ma, Jiangli Dong, Gchen Jin, Daofeng Li, Changwang Deng, Tao Wang

Research output: Contribution to journalArticlepeer-review

61 Scopus citations

Abstract

The multifunctional protein helper component proteinase (HC-Pro) is thought to interfere with the activity of the 20S proteasome; however, no sites of interaction have been identified for either protein. Here, we first show that the Potato virus Y (PVY) HC-Pro protein can interact with three Arabidopsis 20S proteasome subunits (PAA, PBB, and PBE), using a yeast two-hybrid system and the bimolecular fluorescence complement assay. In addition, yeast two-hybrid analysis of the interaction between several mutant subunits of the 20S proteasome and PVY HC-Pro confirmed that residues 81 to 140 of PAA, 1 to 80 of PBB, and 160 to 274 of PBE are necessary for binding PAA, PBB, and PBE to PVY HC-Pro, respectively. Deletion mutant analysis of PVY HC-Pro showed that the N terminus (residues 1 to 97) is necessary for its interaction with three Arabidopsis 20S proteasome subunits. The ability of HC-Pro to interact and interfere with the activity of the 20S proteasome may help explain the molecular basis of its multifunctional character.

Original languageEnglish
Pages (from-to)12881-12888
Number of pages8
JournalJournal of virology
Volume81
Issue number23
DOIs
StatePublished - Dec 2007

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