hADA3 is required for p53 activity

Ting Wang; Takahiko Kobayashi; Rishu Takimoto; Alec E. Denes; Eric L. Snyder; Wafik S. El-Deiry; Rainer K. Brachmann

doi:10.1093/emboj/20.22.6404

hADA3 is required for p53 activity

Ting Wang, Takahiko Kobayashi, Rishu Takimoto, Alec E. Denes, Eric L. Snyder, Wafik S. El-Deiry, Rainer K. Brachmann

Research output: Contribution to journal › Article › peer-review

74 Scopus citations

Abstract

The tumor suppressor protein p53 is a transcription factor that is frequently mutated in human cancers. In response to DNA damage, p53 protein is stabilized and activated by post-translational modifications that enable it to induce either apoptosis or cell cycle arrest. Using a novel yeast p53 dissociator assay, we identify hADA3, a part of histone acetyltransferase complexes, as an important cofactor for p53 activity. p53 and hADA3 physically interact in human cells. This interaction is enhanced dramatically after DNA damage due to phosphorylation event(s) in the p53 N-terminus. Proper hADA3 function is essential for full transcriptional activity of p53 and p53-mediated apoptosis.

Original language	English
Pages (from-to)	6404-6413
Number of pages	10
Journal	EMBO Journal
Volume	20
Issue number	22
DOIs	https://doi.org/10.1093/emboj/20.22.6404
State	Published - Nov 15 2001

Keywords

Apoptosis
DNA damage
Histone acetyltransferase
hADA3
p53

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10.1093/emboj/20.22.6404

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title = "hADA3 is required for p53 activity",

abstract = "The tumor suppressor protein p53 is a transcription factor that is frequently mutated in human cancers. In response to DNA damage, p53 protein is stabilized and activated by post-translational modifications that enable it to induce either apoptosis or cell cycle arrest. Using a novel yeast p53 dissociator assay, we identify hADA3, a part of histone acetyltransferase complexes, as an important cofactor for p53 activity. p53 and hADA3 physically interact in human cells. This interaction is enhanced dramatically after DNA damage due to phosphorylation event(s) in the p53 N-terminus. Proper hADA3 function is essential for full transcriptional activity of p53 and p53-mediated apoptosis.",

keywords = "Apoptosis, DNA damage, Histone acetyltransferase, hADA3, p53",

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T1 - hADA3 is required for p53 activity

AU - Wang, Ting

AU - Kobayashi, Takahiko

AU - Takimoto, Rishu

AU - Denes, Alec E.

AU - Snyder, Eric L.

AU - El-Deiry, Wafik S.

AU - Brachmann, Rainer K.

PY - 2001/11/15

Y1 - 2001/11/15

N2 - The tumor suppressor protein p53 is a transcription factor that is frequently mutated in human cancers. In response to DNA damage, p53 protein is stabilized and activated by post-translational modifications that enable it to induce either apoptosis or cell cycle arrest. Using a novel yeast p53 dissociator assay, we identify hADA3, a part of histone acetyltransferase complexes, as an important cofactor for p53 activity. p53 and hADA3 physically interact in human cells. This interaction is enhanced dramatically after DNA damage due to phosphorylation event(s) in the p53 N-terminus. Proper hADA3 function is essential for full transcriptional activity of p53 and p53-mediated apoptosis.

AB - The tumor suppressor protein p53 is a transcription factor that is frequently mutated in human cancers. In response to DNA damage, p53 protein is stabilized and activated by post-translational modifications that enable it to induce either apoptosis or cell cycle arrest. Using a novel yeast p53 dissociator assay, we identify hADA3, a part of histone acetyltransferase complexes, as an important cofactor for p53 activity. p53 and hADA3 physically interact in human cells. This interaction is enhanced dramatically after DNA damage due to phosphorylation event(s) in the p53 N-terminus. Proper hADA3 function is essential for full transcriptional activity of p53 and p53-mediated apoptosis.

KW - Apoptosis

KW - DNA damage

KW - Histone acetyltransferase

KW - hADA3

KW - p53

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U2 - 10.1093/emboj/20.22.6404

DO - 10.1093/emboj/20.22.6404

M3 - Article

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SN - 0261-4189

VL - 20

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EP - 6413

JO - EMBO Journal

JF - EMBO Journal

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ER -

hADA3 is required for p53 activity

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