TY - JOUR
T1 - Gut microbiota and overweight in 3-year old children
AU - Karvonen, Anne M.
AU - Sordillo, Joanne E.
AU - Gold, Diane R.
AU - Bacharier, Leonard B.
AU - O’Connor, George T.
AU - Zeiger, Robert S.
AU - Beigelman, Avraham
AU - Weiss, Scott T.
AU - Litonjua, Augusto A.
N1 - Funding Information:
Acknowledgements We thank families who have taken part of the VDAART study. We would like to thank Nancy Laranjo for data management; John Ziniti and Nicholas Wolfe for helping with the microbiota data and advanced statistical analyses; Pauli Tuoresmäki for helping with the figure. We also thank the project coordinator, study coordinators and research assistants at the VDAART clinical centers (at Washington University St. Louis, Boston University Medical Center and Kaiser Permanente Southern California). This study was supported by NIH grants HL091528, HL108818 and 1UL1TR001430, and the research grants from the Academy of Finland (grant 287675); the Juho Vainio Foundation; the Foundation for Pediatric Research; and by the National Institute for Health and Welfare, Finland.
Publisher Copyright:
© 2018, Springer Nature Limited.
PY - 2019/4/1
Y1 - 2019/4/1
N2 - Background: The gut microbiota has been associated with overweight and obesity in adults, but the evidence in children is limited. Our aim was to study whether composition of the gut microbiota at the age of 3 years is associated with overweight/obesity in children cross-sectionally. Methods: Children, who participated in a clinical trial of prenatal vitamin-D supplementation (VDAART), underwent standardized height and weight measurements, and collection of stool samples at 3 years of age. 16 S rRNA sequencing (V4 region) of the stool samples were performed with Illumina MiSeq. Associations between microbiota and overweight/obesity (body mass index z-scores >85th percentile) was analyzed using logistic regression. Results: Out of 502 children, 146 (29%) were categorized as overweight/obese. Maternal pre-pregnancy BMI, birth weight and length, formula feeding during the first year, high frequency of fast food consumption, and time watching TV or computer screen at 3 years were the risk factors for overweight/obesity. Of the top 20 most abundant genera, high relative abundance of Parabacteroidetes (Bacteroidetes; Bacteroidales) (aOR(95% CI): 0.69 (0.53, 0.90, p = 0.007) per interquartile increase) and unassigned genus within Peptostreptococcae family were inversely associated with overweight/obesity, whereas high relative abundance of Dorea (Firmicutes;Clostridiales) (1.23 (1.05, 1.43, p = 0.009)) was positively associated. Associations were independent of each other. No associations were found between diversity indices and overweight/obesity. Conclusions: Our data suggest that some of the differences in gut composition of bacteria between obese and non-obese adults can already be observed in 3-year old children. Longitudinal studies will be needed to determine long-term effects.
AB - Background: The gut microbiota has been associated with overweight and obesity in adults, but the evidence in children is limited. Our aim was to study whether composition of the gut microbiota at the age of 3 years is associated with overweight/obesity in children cross-sectionally. Methods: Children, who participated in a clinical trial of prenatal vitamin-D supplementation (VDAART), underwent standardized height and weight measurements, and collection of stool samples at 3 years of age. 16 S rRNA sequencing (V4 region) of the stool samples were performed with Illumina MiSeq. Associations between microbiota and overweight/obesity (body mass index z-scores >85th percentile) was analyzed using logistic regression. Results: Out of 502 children, 146 (29%) were categorized as overweight/obese. Maternal pre-pregnancy BMI, birth weight and length, formula feeding during the first year, high frequency of fast food consumption, and time watching TV or computer screen at 3 years were the risk factors for overweight/obesity. Of the top 20 most abundant genera, high relative abundance of Parabacteroidetes (Bacteroidetes; Bacteroidales) (aOR(95% CI): 0.69 (0.53, 0.90, p = 0.007) per interquartile increase) and unassigned genus within Peptostreptococcae family were inversely associated with overweight/obesity, whereas high relative abundance of Dorea (Firmicutes;Clostridiales) (1.23 (1.05, 1.43, p = 0.009)) was positively associated. Associations were independent of each other. No associations were found between diversity indices and overweight/obesity. Conclusions: Our data suggest that some of the differences in gut composition of bacteria between obese and non-obese adults can already be observed in 3-year old children. Longitudinal studies will be needed to determine long-term effects.
UR - http://www.scopus.com/inward/record.url?scp=85058839337&partnerID=8YFLogxK
U2 - 10.1038/s41366-018-0290-z
DO - 10.1038/s41366-018-0290-z
M3 - Article
C2 - 30568265
AN - SCOPUS:85058839337
SN - 0307-0565
VL - 43
SP - 713
EP - 723
JO - International Journal of Obesity
JF - International Journal of Obesity
IS - 4
ER -