GRK2 moderates the acute mitochondrial damage to ionizing radiation exposure by promoting mitochondrial fission/fusion

Antonietta Franco; Daniela Sorriento; Jessica Gambardella; Roberto Pacelli; Nella Prevete; Claudio Procaccini; Giuseppe Matarese; Bruno Trimarco; Guido Iaccarino; Michele Ciccarelli

doi:10.1038/s41420-018-0028-7

GRK2 moderates the acute mitochondrial damage to ionizing radiation exposure by promoting mitochondrial fission/fusion

Antonietta Franco, Daniela Sorriento, Jessica Gambardella, Roberto Pacelli, Nella Prevete, Claudio Procaccini, Giuseppe Matarese, Bruno Trimarco, Guido Iaccarino, Michele Ciccarelli

Research output: Contribution to journal › Article › peer-review

39 Scopus citations

Abstract

The modern understanding of the G protein-coupled receptor kinase 2 has grown towards the definition of a stress protein, for its ability to rapidly compartmentalize within the cell in response to acute stimulation. Also, mitochondria can be regulated by GRK2 localization. We show that Ionizing Radiation (IR) exposure acutely damages mitochondria regarding mass, morphology, and respiration, with recovery in a framework of hours. This phenomenon is actively regulated by GRK2, whose overexpression results to be protective, and reciprocally, deletion accelerates degenerative processes. The regulatory effects of the kinase involve a new interactome that includes binding HSP90 and binding and phosphorylation of the key molecules involved in the process of mitochondrial fusion and recovery: MFN-1 and 2.

Original language	English
Article number	25
Journal	Cell Death Discovery
Volume	4
Issue number	1
DOIs	https://doi.org/10.1038/s41420-018-0028-7
State	Published - Dec 1 2018

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abstract = "The modern understanding of the G protein-coupled receptor kinase 2 has grown towards the definition of a stress protein, for its ability to rapidly compartmentalize within the cell in response to acute stimulation. Also, mitochondria can be regulated by GRK2 localization. We show that Ionizing Radiation (IR) exposure acutely damages mitochondria regarding mass, morphology, and respiration, with recovery in a framework of hours. This phenomenon is actively regulated by GRK2, whose overexpression results to be protective, and reciprocally, deletion accelerates degenerative processes. The regulatory effects of the kinase involve a new interactome that includes binding HSP90 and binding and phosphorylation of the key molecules involved in the process of mitochondrial fusion and recovery: MFN-1 and 2.",

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AU - Franco, Antonietta

AU - Sorriento, Daniela

AU - Gambardella, Jessica

AU - Pacelli, Roberto

AU - Prevete, Nella

AU - Procaccini, Claudio

AU - Matarese, Giuseppe

AU - Trimarco, Bruno

AU - Iaccarino, Guido

AU - Ciccarelli, Michele

PY - 2018/12/1

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AB - The modern understanding of the G protein-coupled receptor kinase 2 has grown towards the definition of a stress protein, for its ability to rapidly compartmentalize within the cell in response to acute stimulation. Also, mitochondria can be regulated by GRK2 localization. We show that Ionizing Radiation (IR) exposure acutely damages mitochondria regarding mass, morphology, and respiration, with recovery in a framework of hours. This phenomenon is actively regulated by GRK2, whose overexpression results to be protective, and reciprocally, deletion accelerates degenerative processes. The regulatory effects of the kinase involve a new interactome that includes binding HSP90 and binding and phosphorylation of the key molecules involved in the process of mitochondrial fusion and recovery: MFN-1 and 2.

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GRK2 moderates the acute mitochondrial damage to ionizing radiation exposure by promoting mitochondrial fission/fusion

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