TY - JOUR
T1 - Gonadotropin and lipoprotein receptor levels in rat luteal cells of early pregnancy
AU - Wiest, Walter G.
AU - Nakamura, Yukio
AU - Puri, Vipla
AU - Nelson, D. Michael
N1 - Funding Information:
*Presented in part at the 28th annual meeting of the Society for Gynecologic Investigation held in St Louis, Mar. 18-21. 198i. Abst. 223. Miss Toshiko Takemura’s participation in this portion is gratefully acknowledged. Supported by NIH HD 13259. $To whom reprint requests should be addressed.
PY - 1983/7
Y1 - 1983/7
N2 - Dispersed cells from rat corpora lutea isolated on pregnancy days, 5, 7, 10 and 13, yielded three distinct sub-populations of luteal cells when centrifuged in a continuous, 0-40%, Metrizamide density gradient. Bands I and II (refract, ind. 1.340-1.346) constituted highly enriched, functional, luteal cell fractions. The more dense fraction (Band III, refract, ind. 1.348-1.355), while contaminated with non-luteal cells, also contained cells capable of progesterone production. Progesterone synthesis by all three sub-populations was stimulated by LH, and each sub-population bound [125I]-labeled hCG and [125I]-labeled HDL with high affinity. However, the concentrations of LH/hCG and lipoprotein binding sites were greater in Band I + II than in Band III. Progesterone secretion during the selected days of pregnancy, being high on days 5 and 13 with a nadir on day 10, was shown to correlate with the concentration of LH/hCG-binding sites present on Band I + II cells. This correlation was not seen when comparing Band III cells. The concentration of HDL-binding sites did not vary significantly with the sub-populations of luteal cells isolated on the selected days of pregnancy.
AB - Dispersed cells from rat corpora lutea isolated on pregnancy days, 5, 7, 10 and 13, yielded three distinct sub-populations of luteal cells when centrifuged in a continuous, 0-40%, Metrizamide density gradient. Bands I and II (refract, ind. 1.340-1.346) constituted highly enriched, functional, luteal cell fractions. The more dense fraction (Band III, refract, ind. 1.348-1.355), while contaminated with non-luteal cells, also contained cells capable of progesterone production. Progesterone synthesis by all three sub-populations was stimulated by LH, and each sub-population bound [125I]-labeled hCG and [125I]-labeled HDL with high affinity. However, the concentrations of LH/hCG and lipoprotein binding sites were greater in Band I + II than in Band III. Progesterone secretion during the selected days of pregnancy, being high on days 5 and 13 with a nadir on day 10, was shown to correlate with the concentration of LH/hCG-binding sites present on Band I + II cells. This correlation was not seen when comparing Band III cells. The concentration of HDL-binding sites did not vary significantly with the sub-populations of luteal cells isolated on the selected days of pregnancy.
UR - http://www.scopus.com/inward/record.url?scp=0020792160&partnerID=8YFLogxK
U2 - 10.1016/0022-4731(83)90014-6
DO - 10.1016/0022-4731(83)90014-6
M3 - Article
C2 - 6310257
AN - SCOPUS:0020792160
VL - 19
SP - 795
EP - 803
JO - Journal of Steroid Biochemistry
JF - Journal of Steroid Biochemistry
SN - 0022-4731
IS - 1 PART 3
ER -