TY - JOUR
T1 - GmoDetector
T2 - An accurate and efficient GMO identification approach and its applications
AU - Chen, Lihong
AU - Zhou, Junfei
AU - Li, Tiantian
AU - Fang, Zhiwei
AU - Li, Lun
AU - Huang, Gang
AU - Gao, Lifen
AU - Zhu, Xiaobo
AU - Zhou, Xusheng
AU - Xiao, Huafeng
AU - Zhang, Jing
AU - Xiong, Qi Jie
AU - Zhang, Jianan
AU - Ma, Aijin
AU - Zhai, Wenxue
AU - Zhang, Weixiong
AU - Peng, Hai
N1 - Publisher Copyright:
© 2021 Elsevier Ltd
PY - 2021/11
Y1 - 2021/11
N2 - The rapid increase of genetically modified organisms (GMOs) entering the food and feed markets, and the contamination of donor (micro)organisms of transgenic elements make it more challenging for the existing GMO detection. In this study, we developed a high-throughput and contamination-removal GMO detection approach named as GmoDetector. GmoDetector targeted 64 common transgenic elements and 76 GMO-specific events collected from 251 singular GM events, and combined with next generation sequencing (NGS) and target enrichment technology to detect various GMOs. As a result, GmoDetector was able to exclude the donor (micro)organism contamination, and detect the authorized and unauthorized GMOs (UGMOs) in any forms of food or feed, such as processed or unprocessed. The sensitivity of GmoDetector is as low as 0.1% (GMO content), which has met the GMO labeling threshold for all countries. Therefore, GmoDetector is a robust tool for accurate and efficient detection of the authorized and UGMOs.
AB - The rapid increase of genetically modified organisms (GMOs) entering the food and feed markets, and the contamination of donor (micro)organisms of transgenic elements make it more challenging for the existing GMO detection. In this study, we developed a high-throughput and contamination-removal GMO detection approach named as GmoDetector. GmoDetector targeted 64 common transgenic elements and 76 GMO-specific events collected from 251 singular GM events, and combined with next generation sequencing (NGS) and target enrichment technology to detect various GMOs. As a result, GmoDetector was able to exclude the donor (micro)organism contamination, and detect the authorized and unauthorized GMOs (UGMOs) in any forms of food or feed, such as processed or unprocessed. The sensitivity of GmoDetector is as low as 0.1% (GMO content), which has met the GMO labeling threshold for all countries. Therefore, GmoDetector is a robust tool for accurate and efficient detection of the authorized and UGMOs.
KW - GMO characteristic fragment
KW - GMO identification
KW - GmoDetector
KW - Next generation sequencing
KW - Probe capture
UR - http://www.scopus.com/inward/record.url?scp=85114747658&partnerID=8YFLogxK
U2 - 10.1016/j.foodres.2021.110662
DO - 10.1016/j.foodres.2021.110662
M3 - Article
C2 - 34600664
AN - SCOPUS:85114747658
SN - 0963-9969
VL - 149
JO - Food Research International
JF - Food Research International
M1 - 110662
ER -